Cloned (Comment) | Organism |
---|---|
gene hypRE, encoded in the Hyp catabolism locus, DNA and amino acid sequence determination and analysis, expression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21/DE3/pET28 from the T7 promoter | Sinorhizobium meliloti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Sinorhizobium meliloti | |
8 | - |
cis-4-hydroxy-D-proline | pH not specified in the publication, 30°C, recombinant enzyme | Sinorhizobium meliloti | |
10 | - |
trans-4-hydroxy-L-proline | pH not specified in the publication, 30°C, recombinant enzyme | Sinorhizobium meliloti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-4-hydroxy-L-proline | Sinorhizobium meliloti | - |
cis-4-hydroxy-D-proline | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
gene hypRE or smb20268 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-4-hydroxy-L-proline | - |
Sinorhizobium meliloti | cis-4-hydroxy-D-proline | - |
r |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxyproline 2-epimerase | - |
Sinorhizobium meliloti |
HyPRE | - |
Sinorhizobium meliloti |
Smb20268 | - |
Sinorhizobium meliloti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | assay at | Sinorhizobium meliloti |
Organism | Comment | Expression |
---|---|---|
Sinorhizobium meliloti | the enzyme is strongly induced by cis-4-hydroxy-D-proline, moderately by trans-4-hydroxy-L-proline | up |
General Information | Comment | Organism |
---|---|---|
malfunction | a hypRE mutant grows with cis-4-hydroxy-D-proline but not with trans-4-hydroxy-L-proline, and it exhibits wild-type growth on succinate and proline, mutant complementation with the native smb20268 gene | Sinorhizobium meliloti |
metabolism | the enzyme performs the first step of hydroxyproline catabolism, regulation, hyp cluster promoters are regulated by HypR, overview. cis-4-Hydroxy-D-proline is the effector that interacts with HypR and the results suggest a role of positive feedback in the regulation of hyp catabolism, as cis-4-hydroxy-D-proline (or a downstream catabolite) is a stronger inducer than the initial substrate trans-4-hydroxy-L-proline | Sinorhizobium meliloti |
additional information | the reaction mechanism requires two active site cysteines | Sinorhizobium meliloti |
physiological function | the enzyme is absolutely required for growth on trans-4-hydroxy-L-proline | Sinorhizobium meliloti |