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Literature summary for 5.1.1.8 extracted from

  • White, C.E.; Gavina, J.M.; Morton, R.; Britz-McKibbin, P.; Finan, T.M.
    Control of hydroxyproline catabolism in Sinorhizobium meliloti (2012), Mol. Microbiol., 85, 1133-1147.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene hypRE, encoded in the Hyp catabolism locus, DNA and amino acid sequence determination and analysis, expression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21/DE3/pET28 from the T7 promoter Sinorhizobium meliloti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Sinorhizobium meliloti
8
-
cis-4-hydroxy-D-proline pH not specified in the publication, 30°C, recombinant enzyme Sinorhizobium meliloti
10
-
trans-4-hydroxy-L-proline pH not specified in the publication, 30°C, recombinant enzyme Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans-4-hydroxy-L-proline Sinorhizobium meliloti
-
cis-4-hydroxy-D-proline
-
r

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti
-
gene hypRE or smb20268
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-4-hydroxy-L-proline
-
Sinorhizobium meliloti cis-4-hydroxy-D-proline
-
r

Synonyms

Synonyms Comment Organism
4-hydroxyproline 2-epimerase
-
Sinorhizobium meliloti
HyPRE
-
Sinorhizobium meliloti
Smb20268
-
Sinorhizobium meliloti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30 assay at Sinorhizobium meliloti

Expression

Organism Comment Expression
Sinorhizobium meliloti the enzyme is strongly induced by cis-4-hydroxy-D-proline, moderately by trans-4-hydroxy-L-proline up

General Information

General Information Comment Organism
malfunction a hypRE mutant grows with cis-4-hydroxy-D-proline but not with trans-4-hydroxy-L-proline, and it exhibits wild-type growth on succinate and proline, mutant complementation with the native smb20268 gene Sinorhizobium meliloti
metabolism the enzyme performs the first step of hydroxyproline catabolism, regulation, hyp cluster promoters are regulated by HypR, overview. cis-4-Hydroxy-D-proline is the effector that interacts with HypR and the results suggest a role of positive feedback in the regulation of hyp catabolism, as cis-4-hydroxy-D-proline (or a downstream catabolite) is a stronger inducer than the initial substrate trans-4-hydroxy-L-proline Sinorhizobium meliloti
additional information the reaction mechanism requires two active site cysteines Sinorhizobium meliloti
physiological function the enzyme is absolutely required for growth on trans-4-hydroxy-L-proline Sinorhizobium meliloti