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Literature summary for 5.1.1.8 extracted from

  • Gavina, J.M.; White, C.E.; Finan, T.M.; Britz-McKibbin, P.
    Determination of 4-hydroxyproline-2-epimerase activity by capillary electrophoresis: A stereoselective platform for inhibitor screening of amino acid isomerases (2010), Electrophoresis, 31, 2831-2837.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by 5 mM trans-3-hydroxy-L-proline, P5C, and L-proline Pseudomonas aeruginosa
pyrrole 2-carboxylic acid selective inhibitor, competitive inhibition Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
trans-4-hydroxy-D-proline in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C Pseudomonas aeruginosa
0.0024
-
trans-4-hydroxy-L-proline in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cobalt resin column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-4-hydroxy-D-proline
-
Pseudomonas aeruginosa cis-4-hydroxy-L-proline
-
r
trans-4-hydroxy-L-proline
-
Pseudomonas aeruginosa cis-4-hydroxy-D-proline
-
r

Synonyms

Synonyms Comment Organism
4-hydroxyproline-2-epimerase
-
Pseudomonas aeruginosa
HyPRE
-
Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme is pyridoxal 5'-phosphate-independent Pseudomonas aeruginosa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3
-
in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C Pseudomonas aeruginosa pyrrole 2-carboxylic acid