Literature summary for 5.1.1.8 extracted from

Goytia, M.; Chamond, N.; Cosson, A.; Coatnoan, N.; Hermant, D.; Berneman, A.; Minoprio, P.
Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens (2007), PLoS ONE, 2, e885.

Search for more literature for 5.1.1.8

Protein Variants

Protein Variants	Comment	Organism
C236S	radical loss of OH-L/D-Pro epimerization	Pseudomonas aeruginosa
C88S	radical loss of OH-L/D-Pro epimerization	Pseudomonas aeruginosa
V60F	radical loss of OH-L/D-Pro epimerization. V60 residue accounts for OH-Pro ligand specificity	Pseudomonas aeruginosa
V60G	almost complete loss of epimerization of trans-4-hydroxy-L-Pro, some residual activity on trans-4-hydroxy-D-Pro. V60 residue accounts for OH-Pro ligand specificity	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
7.6	-	trans-4-hydroxy-L-Pro	37°C	Brucella suis
9	-	trans-4-hydroxy-L-Pro	37°C	Brucella melitensis
9.8	-	trans-4-hydroxy-L-Pro	37°C	Brucella abortus
14.4	-	trans-4-hydroxy-L-Pro	37°C	Burkholderia pseudomallei
14.4	-	trans-4-hydroxy-L-Pro	37°C	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Brucella abortus	Q57B94	-	-
Brucella melitensis	Q8YJ29	-	-
Brucella melitensis 16M	Q8YJ29	-	-
Brucella suis	Q8FYS0	-	-
Burkholderia pseudomallei	Q63NG7	-	-
Pseudomonas aeruginosa	Q9I476	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trans-4-hydroxy-D-Pro	-	Pseudomonas aeruginosa	cis-4-hydroxy-L-Pro	-	?
trans-4-hydroxy-L-Pro	-	Brucella abortus	cis-4-hydroxy-D-Pro	-	?
trans-4-hydroxy-L-Pro	-	Brucella melitensis	cis-4-hydroxy-D-Pro	-	?
trans-4-hydroxy-L-Pro	-	Brucella suis	cis-4-hydroxy-D-Pro	-	?
trans-4-hydroxy-L-Pro	-	Burkholderia pseudomallei	cis-4-hydroxy-D-Pro	-	?
trans-4-hydroxy-L-Pro	-	Pseudomonas aeruginosa	cis-4-hydroxy-D-Pro	-	?
trans-4-hydroxy-L-Pro	-	Brucella melitensis 16M	cis-4-hydroxy-D-Pro	-	?

Synonyms

Synonyms	Comment	Organism
HyPRE	-	Brucella abortus
PrpA	-	Brucella abortus

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Release 2023.1 (January 2023)