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Literature summary for 5.1.1.8 extracted from

  • Adams, E.; Norton, I.L.
    Purification and properties of inducible hydroxyproline 2-epimerase from Pseudomonas (1964), J. Biol. Chem., 239, 1525-1535.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information pyridoxal 5'-phosphate is not involved in the reaction Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Pseudomonas putida
2,2'-dipyridyl
-
Pseudomonas putida
Acriflavin
-
Pseudomonas putida
AgNO3
-
Pseudomonas putida
Atebrin
-
Pseudomonas putida
CdCl2
-
Pseudomonas putida
CuSO4
-
Pseudomonas putida
HgCl2
-
Pseudomonas putida
hydrazine
-
Pseudomonas putida
hydroxylamine
-
Pseudomonas putida
iodoacetate
-
Pseudomonas putida
KCN
-
Pseudomonas putida
NaAsO2
-
Pseudomonas putida
NEM
-
Pseudomonas putida
p-chloromercuribenzoate
-
Pseudomonas putida
Semicarbazide
-
Pseudomonas putida
ZnCl2
-
Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
22
-
hydroxy-L-Pro
-
Pseudomonas putida
53
-
allohydroxy-L-Pro
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17930
-
low speed sedimentation without reaching equilibrium Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Hydroxy-L-Pro Pseudomonas putida trans-hydroxy-L-Pro or cis-hydroxy-L-Pro, initial reaction of the pathway of hydroxyproline metabolism ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Reaction

Reaction Comment Organism Reaction ID
trans-4-Hydroxy-L-proline = cis-4-hydroxy-D-proline one hydrogen in the substrate is labilized during the reaction Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100
-
-
Pseudomonas putida

Storage Stability

Storage Stability Organism
-15°C, concentrated purified enzyme is stable for at least several weeks Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Allohydroxy-D-Pro
-
Pseudomonas putida Allohydroxy-L-Pro
-
?
D-Ala at 1-2% of the activity relative to allo-D-hydroxy-proline epimerization Pseudomonas putida L-Ala
-
?
Hydroxy-L-Pro trans-hydroxy-L-Pro or cis-hydroxy-L-Pro, initial reaction of the pathway of hydroxyproline metabolism Pseudomonas putida ?
-
?
trans-4-hydroxy-L-Pro
-
Pseudomonas putida cis-4-hydroxy-D-Pro
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8 allohydroxy-L-proline Pseudomonas putida
8
-
hydroxy-L-proline Pseudomonas putida