Literature summary for 5.1.1.7 extracted from

Park, J.; Lee, W.; Song, J.; Kim, S.; Lee, J.; Cheong, C.; Kim, H.
Purification, crystallization and preliminary X-ray crystallographic analysis of diaminopimelate epimerase from Acinetobacter baumannii (2013), Acta Crystallogr. Sect. F, 69, 42-44.

Cloned(Commentary)

Cloned (Comment)	Organism
gene dapF, overexpression of N-terminally His6-tagged enzyme, cloned with a a thrombin cleavage site, in Escherichia coli strain SoluBL21	Acinetobacter baumannii

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 14 mg/ml protein in 20 mM HEPES, pH 7.0, 100 mM NaCl, and 5 mM DTT, with 0.001 ml of reservoir solution containing 2 M ammonium sulfate, 0.1 M sodium HEPES, pH 7.5, and 4.9-5.1% PEG 400, equilibration against reservoir solution, 25% v/v PEG 400 as a cryoprotectant, method optimization, X-ray diffraction structure determination and analysis at 1.9 A resolution	Acinetobacter baumannii

Crystallization (Comment)

Organism

purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 14 mg/ml protein in 20 mM HEPES, pH 7.0, 100 mM NaCl, and 5 mM DTT, with 0.001 ml of reservoir solution containing 2 M ammonium sulfate, 0.1 M sodium HEPES, pH 7.5, and 4.9-5.1% PEG 400, equilibration against reservoir solution, 25% v/v PEG 400 as a cryoprotectant, method optimization, X-ray diffraction structure determination and analysis at 1.9 A resolution

Acinetobacter baumannii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31100	-	x * 31100	Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
LL-2,6-Diaminoheptanedioate	Acinetobacter baumannii	-	meso-Diaminoheptanedioate	-	r

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter baumannii	-	gene dapF	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia coli strain SoluBL21 by nickel affinity chromatography, tag cleavage by thrombin, another step of nickel affinity chromatography, ultrafiltration, and gel filtration	Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
LL-2,6-Diaminoheptanedioate	-	Acinetobacter baumannii	meso-Diaminoheptanedioate	-	r

Subunits

Subunits	Comment	Organism
?	x * 31100	Acinetobacter baumannii

Synonyms

Synonyms	Comment	Organism
DapF	-	Acinetobacter baumannii

Cofactor

Cofactor	Comment	Organism	Structure
additional information	the enzyme is independent of pyridoxal 5'-phosphate	Acinetobacter baumannii

General Information

General Information	Comment	Organism
evolution	the enzyme is a member of the pyridoxal 5'-phosphate-independent racemase family of enzymes	Acinetobacter baumannii
metabolism	meso-diaminopimelate is a biosynthetic precursor of L-lysine in bacteria	Acinetobacter baumannii
physiological function	(2R,6S)-2,6-diaminopimelic acid, i.e. meso-diaminopimelate, in the pentapeptide of cell wall peptidoglycan provides the attachment site for the inner or outer membrane to peptidoglycan	Acinetobacter baumannii