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Literature summary for 5.1.1.5 extracted from

  • Kato, S.; Hemmi, H.; Yoshimura, T.
    Lysine racemase from a lactic acid bacterium, Oenococcus oeni: structural basis of substrate specificity (2012), J. Biochem., 152, 505-508.
    View publication on PubMed
Search for more literature for 5.1.1.5

Cloned(Commentary)

Cloned (Comment) Organism
gene OEOE0162, DNA and amino acid sequence determination and analysis, expression of the enzyme in Escherichia coli strain BL21 Oenococcus oeni

Protein Variants

Protein Variants Comment Organism
T224I site-directed mutagenesis, the mutant shows significantly decreased activity compared to the wild-type enzyme Oenococcus oeni
T224I/W355Y site-directed mutagenesis, the double mutant shows significantly decreased activity compared to the wild-type enzyme Oenococcus oeni
W355Y site-directed mutagenesis, the mutant shows significantly decreased activity compared to the wild-type enzyme Oenococcus oeni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1 2 D-Lysine pH 8.0, 30°C, recombinant mutant T224I Oenococcus oeni
2 3 L-lysine pH 8.0, 30°C, recombinant mutant W355Y Oenococcus oeni
9.8
-
 D-Lysine pH 8.0, 30°C, recombinant wild-type enzyme Oenococcus oeni
11
-
 L-lysine pH 8.0, 30°C, recombinant wild-type enzyme Oenococcus oeni
15
-
 L-lysine pH 8.0, 30°C, recombinant mutant T224I Oenococcus oeni
21
-
 D-Lysine pH 8.0, 30°C, recombinant mutant W355Y Oenococcus oeni
22
-
 D-ornithine pH 8.0, 30°C, recombinant wild-type enzyme Oenococcus oeni
27
-
 L-ornithine pH 8.0, 30°C, recombinant wild-type enzyme Oenococcus oeni
27
-
 L-lysine pH 8.0, 30°C, recombinant mutant T224I/W355Y Oenococcus oeni
31
-
 D-Lysine pH 8.0, 30°C, recombinant mutant T224I/W355Y Oenococcus oeni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine Oenococcus oeni
-
 D-lysine
-
 ?
additional information Oenococcus oeni the Oenococcus oeni lysine racemase has a specific activity towards basic amino acids, residues Thr224 and Trp355 are important for enzyme substrate specificity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oenococcus oeni Q04HB7 gene OEOE0162
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-lysine
-
 Oenococcus oeni L-lysine
-
 r
D-ornithine
-
 Oenococcus oeni L-ornithine
-
 r
L-lysine
-
 Oenococcus oeni D-lysine
-
 ?
L-lysine
-
 Oenococcus oeni D-lysine
-
 r
L-Ornithine
-
 Oenococcus oeni D-Ornithine
-
 r
additional information the Oenococcus oeni lysine racemase has a specific activity towards basic amino acids, residues Thr224 and Trp355 are important for enzyme substrate specificity Oenococcus oeni ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oenococcus oeni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 D-Lysine pH 8.0, 30°C, recombinant mutant T224I/W355Y Oenococcus oeni
0.16
-
 L-lysine pH 8.0, 30°C, recombinant mutant T224I/W355Y Oenococcus oeni
0.28
-
 D-Lysine pH 8.0, 30°C, recombinant mutant T224I Oenococcus oeni
0.32
-
 L-lysine pH 8.0, 30°C, recombinant mutant T224I Oenococcus oeni
0.88
-
 D-Lysine pH 8.0, 30°C, recombinant mutant W355Y Oenococcus oeni
1.03
-
 L-lysine pH 8.0, 30°C, recombinant mutant W355Y Oenococcus oeni
1.83
-
 L-ornithine pH 8.0, 30°C, recombinant wild-type enzyme Oenococcus oeni
1.83
-
 D-ornithine pH 8.0, 30°C, recombinant wild-type enzyme Oenococcus oeni
2.83
-
 D-Lysine pH 8.0, 30°C, recombinant wild-type enzyme Oenococcus oeni
3.5
-
 L-lysine pH 8.0, 30°C, recombinant wild-type enzyme Oenococcus oeni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Oenococcus oeni

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Oenococcus oeni

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.005
-
 D-Lysine pH 8.0, 30°C, recombinant mutant T224I/W355Y Oenococcus oeni
0.006
-
 L-lysine pH 8.0, 30°C, recombinant mutant T224I/W355Y Oenococcus oeni
0.023
-
 D-Lysine pH 8.0, 30°C, recombinant mutant T224I Oenococcus oeni
0.025
-
 L-lysine pH 8.0, 30°C, recombinant mutant T224I Oenococcus oeni
0.042
-
 D-Lysine pH 8.0, 30°C, recombinant mutant W355Y Oenococcus oeni
0.045
-
 L-lysine pH 8.0, 30°C, recombinant mutant W355Y Oenococcus oeni
0.07
-
 L-ornithine pH 8.0, 30°C, recombinant wild-type enzyme Oenococcus oeni
0.08
-
 D-ornithine pH 8.0, 30°C, recombinant wild-type enzyme Oenococcus oeni
0.28
-
 D-Lysine pH 8.0, 30°C, recombinant wild-type enzyme Oenococcus oeni
0.32
-
 L-lysine pH 8.0, 30°C, recombinant wild-type enzyme Oenococcus oeni