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Literature summary for 5.1.1.5 extracted from

  • Chen, I.; Lin, W.; Hsu, S.; Thiruvengadam, V.; Hsu, W.
    Isolation and characterization of a novel lysine racemase from a soil metagenomic library (2009), Appl. Environ. Microbiol., 75, 5161-5166.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
dipicolinic acid complete inhibition by metal-chelating agents in 50 mM Tris-HCl buffer (pH 8.0) at 30°C uncultured bacterium
EDTA complete inhibition by metal-chelating agents in 50 mM Tris-HCl buffer (pH 8.0) at 30°C uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
16.21
-
 L-lysine in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C uncultured bacterium
23.48
-
 D-Lysine in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ addition of 2 mM extrinsic divalent ions enhances the enzyme activity up to 114.5% uncultured bacterium
Mg2+ addition of 2 mM extrinsic divalent ions enhances the enzyme activity up to 83.6 uncultured bacterium
Mn2+ addition of 2 mM extrinsic divalent ions enhances the enzyme activity up to 97.3% uncultured bacterium
Zn2+ analyzing of bound metal ions in purified Lyr with an inductively coupled plasma spectrometer shows that dialyzed Lyr contains 0.77 mol of Zn2+ per mol of the dimeric enzyme, while cobalt, magnesium, manganese, nickel, or copper ions are absent uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42700
-
 protein composed of 393 amino acids with the calculated molecular mass, confirmed by SDS-PAGE uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine uncultured bacterium
-
 D-lysine enzyme exhibits higher specific activity toward lysine in the L-lysine to D-lysine direction than in the reverse reaction r

Organism

Organism UniProt Comment Textmining
uncultured bacterium C7ACH5 from garden soil, the lyr gene may have only recently evolved from Escherichia coli N-acetyl-gamma-glutamyl-phosphate reductase and Deinococcus radiodurans N-acyl amino acid racemase, the partial regions of both enzymes might have been combined to evolve a novel enzyme, in which the new catalytic sites are created for catalyzing the racemization of lysine
-

Purification (Commentary)

Purification (Comment) Organism
by nickel chelate affinity chromatography uncultured bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.68
-
 substrate 10 mM D-lysine, in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C uncultured bacterium
3.61
-
 substrate 10 mM L-lysine, in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine
-
 uncultured bacterium D-lysine enzyme exhibits higher specific activity toward lysine in the L-lysine to D-lysine direction than in the reverse reaction r

Synonyms

Synonyms Comment Organism
lyr
-
 uncultured bacterium
lysine racemase
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 maximum activity uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme shows considerable stability at 50°C, with a half-life of about 3 days uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.16
-
 D-Lysine in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C uncultured bacterium
5.1
-
 L-lysine in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 50 mM Tris-HCl uncultured bacterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 about 15% of maximal activity is observed at pHs of below 5 and above 9 uncultured bacterium