Application | Comment | Organism |
---|---|---|
drug development | enzyme has potential to be used as a drug target for this parasite-based trypanosomiasis | Trypanosoma vivax |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) with a C-terminal 6-His tag | Trypanosoma vivax |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyrrole-2-carboxylic acid | competitive inhibitor | Trypanosoma vivax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
106 | - |
D-proline | 40 mM substrate in 0.2 M sodium acetate, for 30 min at 37°C | Trypanosoma vivax | |
145 | - |
L-proline | 40 mM substrate in 0.2 M sodium acetate, for 30 min at 37°C | Trypanosoma vivax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
recombinant enzyme, determined by SDS-PAGE | Trypanosoma vivax |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline | Trypanosoma vivax | - |
D-proline | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma vivax | B8LFE4 | - |
- |
Purification (Comment) | Organism |
---|---|
by affinity chromatography using nickel-nitrilotriacetic acid-agarose columns | Trypanosoma vivax |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bloodstream form | - |
Trypanosoma vivax | - |
epimastigote | - |
Trypanosoma vivax | - |
trypomastigote | - |
Trypanosoma vivax | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline | - |
Trypanosoma vivax | D-proline | - |
r |
Synonyms | Comment | Organism |
---|---|---|
proline racemase | - |
Trypanosoma vivax |
TvHYP1 | - |
Trypanosoma vivax |
TvPRAC | - |
Trypanosoma vivax |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma vivax |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Trypanosoma vivax |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
pyrrole-2-carboxylic acid | 0.005 mM pyrrole-2-carboxylic acid, serial concentrations of 20-160 mM L-proline | Trypanosoma vivax |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is a T-cell-independent B-cell mitogen, enzyme displays mitogenic activity towards splenic cells from euthymic Swiss mice, since addition of 0.1 mg/ml of recombinant protein promotes a 13fold increase of thymidine incorporation when compared with untreated cells, mitogenic activity of recombinant enzyme seems to be dependent on the active enzyme, since inhibition with 10 mM pyrrole-2-carboxylic acid prior to its incubation with splenocytes specifically decreases proliferation by 44%, enzyme triggers high levels of B-cell activation, terminal differentiation and antibody secretion | Trypanosoma vivax |