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Literature summary for 5.1.1.4 extracted from
- Purification and mechanism of action of proline racemase (1968), Biochemistry, 7, 3970-3978.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activates | Acetoanaerobium sticklandii |  |
| 2-mercaptoethanol | optimal concentration: 0.02 M | Acetoanaerobium sticklandii | |
| additional information | addition of pyridoxal 5'-phosphate does not increase activity | Acetoanaerobium sticklandii | |
| NaBH4 | activates | Acetoanaerobium sticklandii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Acetoanaerobium sticklandii | |
| 2-Furoic acid | weak | Acetoanaerobium sticklandii | |
| 2-Thiophenecarboxylic acid | - |
Acetoanaerobium sticklandii | |
| alpha-Pipecoline | - |
Acetoanaerobium sticklandii | |
| Asp | - |
Acetoanaerobium sticklandii | |
| Fumaric acid | - |
Acetoanaerobium sticklandii | |
| HPO42- | no inhibition by H2PO4- | Acetoanaerobium sticklandii | |
| iodoacetamide | - |
Acetoanaerobium sticklandii | |
| iodoacetate | DL-Pro and pyrrole-2-carboxylate can protect | Acetoanaerobium sticklandii | |
| Maleic acid | - |
Acetoanaerobium sticklandii | |
| malonic acid | - |
Acetoanaerobium sticklandii | |
| Methoxyacetic acid | weak | Acetoanaerobium sticklandii | |
| Phthalic acid | - |
Acetoanaerobium sticklandii | |
| pipecolic acid | weak | Acetoanaerobium sticklandii | |
| pyrrole-2-carboxylic acid | - |
Acetoanaerobium sticklandii | |
| sarcosine | - |
Acetoanaerobium sticklandii | |
| succinic acid | - |
Acetoanaerobium sticklandii | |
| Tetrahydrofuroic acid | weak | Acetoanaerobium sticklandii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
L-Pro | - |
Acetoanaerobium sticklandii | |
| 3.8 | - |
D-Pro | - |
Acetoanaerobium sticklandii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetoanaerobium sticklandii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetoanaerobium sticklandii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 600 | - |
- |
Acetoanaerobium sticklandii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| stored 6 months in the refrigerator without significant loss of activity | Acetoanaerobium sticklandii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Pro | - |
Acetoanaerobium sticklandii | D-Pro | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half-life: 3.6 min, in D2O 17 min | Acetoanaerobium sticklandii |
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Release 2023.1 (January 2023)
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