Cloned (Comment) | Organism |
---|---|
identification of two insertions in gene racE1, suggesting that its glutamate racemase product is not essential for Bacillus anthracis growth | Bacillus anthracis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | using allelic replacement, chromosomal racE2 is replaced with the spectinomycin resistance gene (aad9) and transduced via bacteriophage CP-51 into Bacillus anthracis strain Sterne, generating strain SYO2 (DELTAracE2::aad9) | Bacillus anthracis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Bacillus anthracis | - |
D-glutamate | - |
r | |
L-glutamate | Bacillus anthracis Sterne 34F2 | - |
D-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | Q81LA8 | - |
- |
Bacillus anthracis | Q81UL8 | - |
- |
Bacillus anthracis Sterne 34F2 | Q81LA8 | - |
- |
Bacillus anthracis Sterne 34F2 | Q81UL8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Bacillus anthracis | D-glutamate | - |
r | |
L-glutamate | - |
Bacillus anthracis Sterne 34F2 | D-glutamate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
BAS0806 | - |
Bacillus anthracis |
BAS4379 | - |
Bacillus anthracis |
GBAA_0847 | - |
Bacillus anthracis |
GBAA_4717 | - |
Bacillus anthracis |
MurI | - |
Bacillus anthracis |
RacE1 | - |
Bacillus anthracis |
RacE2 | - |
Bacillus anthracis |
General Information | Comment | Organism |
---|---|---|
malfunction | the racE1 deletion does not affect growth rates, whereas racE2 deletion delays the vegetative growth of Bacillus anthracis following spore germination, producing aberrant cell shapes that can only partially be suppressed with exogenous D-glutamate. Deletion of racE1 or racE2 does not affect the synthesis or stereochemical composition of Bacillus anthracis poly-gamma-D-glutamic acid (PDGA) capsule, and mutants with a deletion of both genes, racE1 and racE2, are not viable | Bacillus anthracis |
physiological function | genes racE1 and racE2, encoding two glutamate racemases, are not essential for growth of Bacillus anthracis noncapsulating vaccine strain Sterne and fully virulent, capsulating strain Ames, even in the absence of exogenous D-glutamate. Modeling of Bacillus anthracis, similar to Bacillus subtilis, utilizing two functionally redundant racemase enzymes to synthesize D-glutamic acid for peptidoglycan synthesis | Bacillus anthracis |
physiological function | genes racE1 and racE2, encoding two glutamate racemases,are not essential for growth of Bacillus anthracis noncapsulating vaccine strain Sterne and fully virulent, capsulating strain Ames, even in the absence of exogenous D-glutamate. Modeling of Bacillus anthracis, similar to Bacillus subtilis, utilizing two functionally redundant racemase enzymes to synthesize D-glutamic acid for peptidoglycan synthesis | Bacillus anthracis |