Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus anthracis |
Protein Variants | Comment | Organism |
---|---|---|
K106A | the isozyme RacE2 mutant is insensitive to dipicolinic acid | Bacillus anthracis |
S207A | the isozyme RacE2 mutant is insensitive to dipicolinic acid | Bacillus anthracis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dipicolinic acid | allosteric inhibitor of both Bacillus anthracis glutamate racemase isozymes which exhibits low micromolar inhibition with clear noncompetitive behavior | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Co2+-affinity column chromatography, and anion-exchange chromatography | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glutamate | - |
Bacillus anthracis | L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RacE1 | isozyme | Bacillus anthracis |
RacE2 | isozyme | Bacillus anthracis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus anthracis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
dipicolinic acid | isozyme RacE1, pH and temperature not specified in the publication | Bacillus anthracis | |
0.092 | - |
dipicolinic acid | isozyme RacE2, pH and temperature not specified in the publication | Bacillus anthracis | |
1.236 | - |
dipicolinic acid | RacE2 mutant enzyme S207A, pH and temperature not specified in the publication | Bacillus anthracis | |
2.342 | - |
dipicolinic acid | RacE2 mutant enzyme K106A, pH and temperature not specified in the publication | Bacillus anthracis |