Literature summary for 5.1.1.3 extracted from

Kim, K.H.; Bong, Y.J.; Park, J.K.; Shin, K.J.; Hwang, K.Y.; Kim, E.E.
Structural basis for glutamate racemase inhibition (2007), J. Mol. Biol., 372, 434-443.

Search for more literature for 5.1.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
cloned in Escherichia coli as a fusion protein with a 6x His tag at the N-terminus	Streptococcus pyogenes

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallization is carried out by the sitting-drop,vapor-diffusion method, crystal structures of GluR from Streptococcus pyogenes in both inhibitor-free and inhibitor-bound forms. The inhibitor-free GluR crystallizes in two different forms, which diffracts to 2.25 A and 2.5 A resolution, while the inhibitor-bound crystal diffracted to 2.5 A resolution.	Streptococcus pyogenes

Inhibitors

Inhibitors	Comment	Organism	Structure
gamma-2-naphthylmethyl-D-glutamate	potent competitive inhibitor that induces a disorder in one of the loops near the active site	Streptococcus pyogenes

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.74	-	L-glutamate	-	Streptococcus pyogenes
126	-	D-glutamate	-	Streptococcus pyogenes

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29000	-	method not indicated	Streptococcus pyogenes

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pyogenes	Q9A1B7	-	-

Purification (Commentary)

Purification (Comment)	Organism
protein extract is purified by using a nickel-chelated Hi-trap chelating column, Hi-trap sp-FF cation-exchange column and by by gel-filtration on a Superdex-200 prep-grade column	Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate	-	Streptococcus pyogenes	D-glutamate	-	r

Subunits

Subunits	Comment	Organism
dimer	crystal structure analysis, GluR is composed of two domains of alpha/beta protein that are related by pseudo-2fold symmetry and the active site is located at the domain interface	Streptococcus pyogenes

Synonyms

Synonyms	Comment	Organism
GluR	-	Streptococcus pyogenes
glutamate racemase	-	Streptococcus pyogenes

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Streptococcus pyogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.49	-	D-glutamate	-	Streptococcus pyogenes
1.63	-	L-glutamate	-	Streptococcus pyogenes

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Streptococcus pyogenes

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)