Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.1.3 extracted from

  • Hwang, K.Y.; Cho, C.S.; Kim, S.S.; Sung, H.C.; Yu, Y.G.; Cho, Y.
    Structure and mechanism of glutamate racemase from Aquifex pyrophilus (1999), Nat. Struct. Biol., 6, 422-426.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, crystal structure of apo-enzyme and enzyme complexed with a substrate analog, D-glutamine, by multiwavelength anomalous dispersion method using a thimerosal-bound MurI crystal, determined at 2.3 A resolution Aquifex pyrophilus

Protein Variants

Protein Variants Comment Organism
D7S turnover number is decreased by 170fold compared to wild-type enzyme in the D to L reaction, it is decreased by about 6fold compared to wild-type enzyme in the L to D reaction Aquifex pyrophilus
E147N turnover number is decreased by 100fold compared to wild-type enzyme in the D to L reaction, it is decreased by about 7fold compared to wild-type enzyme in the L to D reaction Aquifex pyrophilus

Inhibitors

Inhibitors Comment Organism Structure
D-Gln competitive Aquifex pyrophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
D-glutamate pH 8.5, mutant enzyme D7S Aquifex pyrophilus
0.55
-
D-glutamate pH 8.5, wild-type enzyme Aquifex pyrophilus
3
-
D-glutamate pH 8.5, mutant enzyme E147N Aquifex pyrophilus
3.2
-
L-glutamate pH 8.5, mutant enzyme E147N Aquifex pyrophilus
3.3
-
L-glutamate pH 8.5, wild-type enzyme Aquifex pyrophilus
11
-
L-glutamate pH 8.5, mutant enzyme D7S Aquifex pyrophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Aquifex pyrophilus the enzyme is responsible for the synthesis of D-glutamate, an essential building block of the peptidoglycan layer in bacterial cell walls D-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Aquifex pyrophilus P56868
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate the enzyme is responsible for the synthesis of D-glutamate, an essential building block of the peptidoglycan layer in bacterial cell walls Aquifex pyrophilus D-glutamate
-
?

Subunits

Subunits Comment Organism
dimer each monomer consists of two alpha/beta fold domains Aquifex pyrophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00034
-
D-glutamate pH 8.5, mutant enzyme D7S Aquifex pyrophilus
0.0023
-
L-glutamate pH 8.5, mutant enzyme E147N Aquifex pyrophilus
0.0047
-
L-glutamate pH 8.5, mutant enzyme E147N Aquifex pyrophilus
0.037
-
L-glutamate pH 8.5, mutant enzyme D7S Aquifex pyrophilus
0.056
-
D-glutamate pH 8.5, wild-type enzyme Aquifex pyrophilus
0.21
-
L-glutamate pH 8.5, wild-type enzyme Aquifex pyrophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
50
-
D-Gln
-
Aquifex pyrophilus