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Literature summary for 5.1.1.18 extracted from

  • Harty, M.; Nagar, M.; Atkinson, L.; Legay, C.M.; Derksen, D.J.; Bearne, S.L.
    Inhibition of serine and proline racemases by substrate-product analogues (2014), Bioorg. Med. Chem. Lett., 24, 390-393.
    View publication on PubMed
Search for more literature for 5.1.1.18

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
 Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
alpha-(hydroxymethyl)-L-serine substrate-product analogue, a modest, linear mixed-type inhibitor of serine racemase Schizosaccharomyces pombe
additional information substrate-product analogue inhibitors of racemases may only be effective when the active site is capacious and/or plastic, or when the inhibitor is sufficiently flexible Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
19
-
 L-serine pH and temperature not specified in the publication Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Schizosaccharomyces pombe
Mg2+ activates Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine Schizosaccharomyces pombe
-
 D-serine
-
 ?
additional information Schizosaccharomyces pombe the enzyme catalyzes both the racemization and alpha,beta-elimination reaction of L- and D-serine to yield pyruvate and ammonia ?
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-serine = D-serine two-base racemization mechanism, overview. The enzyme utilizes a two-base mechanism wherein one enantiospecific Broensted base abstracts the proton from the L-Ser-pyridoxal 5'-phosphate aldimine and the conjugate acid of a second enantiospecific Bronsted base protonates the intermediate to form the D-Ser-pyridoxal 5'-phosphate aldimine, and vice versa Schizosaccharomyces pombe
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine
-
 Schizosaccharomyces pombe D-serine
-
 ?
additional information the enzyme catalyzes both the racemization and alpha,beta-elimination reaction of L- and D-serine to yield pyruvate and ammonia Schizosaccharomyces pombe ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Schizosaccharomyces pombe

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
167
-
 alpha-(hydroxymethyl)-L-serine pH and temperature not specified in the publication Schizosaccharomyces pombe