Cloned (Comment) | Organism |
---|---|
gene serR, encoding the enzyme, is located on chromosome 4 of the genomic DNA, phylogenetic analysis, cloning and expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | ATP decreases the serine racemase activity of SerR but increases the serine dehydratase activity | Oryza sativa | |
additional information | SerR was inhibited by pyridoxal 5'-phosphate-enzyme inhibitors | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ increases the catalytic efficiency of the serine racemase activity of SerR and decreases that of the serine dehydratase activity. The structure of SerR is distorted by the addition of Mg2+, which is probably involved in the enzyme regulation | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
recombinant enzyme, gel filtration | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | Oryza sativa | - |
D-serine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q7XSN8 | ssp. Japonica cv. Nipponbare or Koshihikari, gene serR | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged SerR 16.9fold from Escherichia coli strain BL21 (DE3) to homogeneity ny nickel affinity chromatography | Oryza sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
purified recombinant enzyme | Oryza sativa |
Storage Stability | Organism |
---|---|
4°C, purified recombinant enzyme, 20 mM sodium phosphate buffer, pH 8.0, 5 days, no loss of activity, but in 20 mM sodium or potassium phosphate buffer, pH 7.0, the enzyme is unstable and becomes inactivated, forming aggregates, leading to loss of about 30% of the initial activity after storage for 3 days | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Oryza sativa | D-serine | - |
r | |
L-serine | SerR is highly specific for serine | Oryza sativa | D-serine | - |
r | |
additional information | in addition to a serine racemase reaction, SerR catalyzes D- and L-serine dehydratase reactions, where the two compounds are converted to pyruvate, overview | Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 x 34500, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
SerR | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Oryza sativa |