Application | Comment | Organism |
---|---|---|
biotechnology | production of beta-lactam antibiotics | Actinomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
isopenicillin N | - |
Actinomyces sp. | |
0.78 | - |
Penicillin N | - |
Actinomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Actinomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopenicillin N | Actinomyces sp. | enzyme epimerizes the side chain of alpha-aminoadipic acid in isopenicillin N from the L- to the D-configuration, this is an important step since the next enzyme in the pathway, DAOCS, is unable to use isopenicillin N as substrate, in spite of the apparent minor structural difference between the two molecules | penicillin N | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by anion exchange chromatography, purified IPN epimerase is able to act in both orientations, leading in vitro to an equimolecular mixture of isopenicillin N and penicillin N | Actinomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopenicillin N | enzyme epimerizes the side chain of alpha-aminoadipic acid in isopenicillin N from the L- to the D-configuration, this is an important step since the next enzyme in the pathway, DAOCS, is unable to use isopenicillin N as substrate, in spite of the apparent minor structural difference between the two molecules | Actinomyces sp. | penicillin N | - |
r |
Synonyms | Comment | Organism |
---|---|---|
isopenicillin N epimerase | - |
Actinomyces sp. |
PenN epimerase | - |
Actinomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
isopenicillin N epimerase, standard assay mixture contains 1.4 mM isopenicillin N or penicillin N, 0.2 mM dithiothreitol, 0.1 mM pyridoxal phosphate and 50 mM pyrophosphate | Actinomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | cofactor of isopenicillin N epimerase is essential for activity and is supposed to bind the conserved amino acid sequence SXHKXL | Actinomyces sp. |