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Literature summary for 5.1.1.17 extracted from
- Purification and properties of isopenicillin-N epimerase from Streptomyces clavuligerus (1989), Biochim. Biophys. Acta, 999, 78-85.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | 1 mM, 88% inhibition | Streptomyces clavuligerus |  |
| D-cycloserine | 1 mM, 23% inhibition | Streptomyces clavuligerus | |
| FAD | 0.5 mM, 80% inhibition | Streptomyces clavuligerus | |
| fluorescamine | inactivation at molar ratios higher than 2 | Streptomyces clavuligerus | |
| hydroxylamine | 1 mM, 95% inhibition | Streptomyces clavuligerus | |
| iodoacetamide | 1 mM, 28% inhibition | Streptomyces clavuligerus | |
| Iproniazid | 1 mM, 25% inhibition | Streptomyces clavuligerus | |
| isoniazid | 1 mM, 23% inhibition | Streptomyces clavuligerus | |
| N-ethylmaleimide | 1 mM, 45% inhibition | Streptomyces clavuligerus | |
| Phenylglyoxal | 1 mM, 33% inhibition | Streptomyces clavuligerus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | - |
pyridoxal 5'-phosphate | - |
Streptomyces clavuligerus | |
| 0.3 | - |
isopenicillin N | - |
Streptomyces clavuligerus | |
| 0.78 | - |
Penicillin N | - |
Streptomyces clavuligerus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
1 * 47000, SDS-PAGE | Streptomyces clavuligerus |
| 50000 | - |
gel filtration | Streptomyces clavuligerus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isopenicillin N | Streptomyces clavuligerus | - |
penicillin N | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces clavuligerus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DE-52, DEAE-Affi-gel blue, Sephadex G-200, calcium phosphate, Mono Q | Streptomyces clavuligerus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.85 | - |
isopenicillin N epimerization | Streptomyces clavuligerus |
| 7.77 | - |
penicillin N epimerization | Streptomyces clavuligerus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deacetoxycephalosporin C | less than 1% of activity with penicillin N | Streptomyces clavuligerus | ? | - |
? | |
| isopenicillin | - |
Streptomyces clavuligerus | penicillin N | - |
r | |
| isopenicillin N | - |
Streptomyces clavuligerus | penicillin N | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000, SDS-PAGE | Streptomyces clavuligerus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
rapid denaturation above | Streptomyces clavuligerus |
| 60 | - |
complete inactivation after 10 min | Streptomyces clavuligerus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.3 | - |
Streptomyces clavuligerus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | 10 | stable at 4°C for 24 h | Streptomyces clavuligerus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 1 mol of pyridoxal 5'-phosphate per mol enzyme, 1.5fold increase in activity in the presence of 0.01 mM pyridoxal 5'-phosphate | Streptomyces clavuligerus | |
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