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Literature summary for 5.1.1.13 extracted from

  • Tanaka-Hayashi, A.; Hayashi, S.; Inoue, R.; Ito, T.; Konno, K.; Yoshida, T.; Watanabe, M.; Yoshimura, T.; Mori, H.
    Is D-aspartate produced by glutamic-oxaloacetic transaminase-1 like 1 (Got1l1) a putative aspartate racemase? (2015), Amino Acids, 47, 79-86 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Got1l1, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21 and in HEK-293T cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information generation of Got1l1 knockout mice. Got1l1 mRNA is highly expressed in the testis in wild-type mice, but is not detected in Got1l1 knockout mice. The D-aspartate contents of knockout and Got1l1 knockout mice are not significantly different in the testis and hippocampus Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Mus musculus 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate Mus musculus
-
D-aspartate
-
r
additional information Mus musculus recombinant Got1l1 protein is a pyridoxal 5'-phosphate-dependent enzyme and synthesizes substantial D-aspartate from L-aspartate and only one-fifth as much L-glutamate, with very little D-glutamate in vitro ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q7TSV6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21 and HEK-293T cells by nickel affinity chromatography and desalting gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
additional information no expression of Got1l1 mRNA in the brain and submandibular gland, where D-aspartate is abundant Mus musculus
-
testis Got1l1 mRNA is highly expressed in the testis Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate
-
Mus musculus D-aspartate
-
r
additional information recombinant Got1l1 protein is a pyridoxal 5'-phosphate-dependent enzyme and synthesizes substantial D-aspartate from L-aspartate and only one-fifth as much L-glutamate, with very little D-glutamate in vitro Mus musculus ?
-
?
additional information recombinant Got1l1 expressed in mammalian cells shows L-aspartate aminotransferase activity, but lacks aspartate racemase activity. When L-aspartate and 2-oxoglutarate are reacted with the Got1l1 purified from HEK293T cells, neither D-aspartate nor D-glutamate is generated, but a detectable amount of L-glutamate is obtained Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
GOT1L1
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Mus musculus

General Information

General Information Comment Organism
malfunction D-aspartate contents of wild-type and Got1l1 knockout mice are not significantly different in the testis and hippocampus. The recombinant Got1l1 expressed in mammalian cells shows L-aspartate aminotransferase activity, but lacks aspartate racemase activity Mus musculus
metabolism enzyme Got1l1 is not the major aspartate racemase, there might be another D-aspartate-synthesizing enzyme Mus musculus
physiological function in the hippocampus, D-aspartate strongly enhances N-methyl-D-aspartate receptor-dependent long-term potentiation and is involved in learning and memory Mus musculus