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Literature summary for 5.1.1.13 extracted from

  • Yamaguchi, T.; Choi, S.Y.; Okada, H.; Yohda, M.; Kumagai, H.; Esaki, N.; Soda, K.
    Properties of aspartate racemase, a pyridoxal 5'-phosphate-independent amino acid racemase (1992), J. Biol. Chem., 267, 18361-18364.
    View publication on PubMed
Search for more literature for 5.1.1.13

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme contains no bound pyridoxal phosphate Streptococcus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
2-Nitro-5-thiocyanobenzoate
-
 Streptococcus thermophilus
5,5'-dithiobis(2-nitrobenzoate)
-
 Streptococcus thermophilus
iodoacetamide
-
 Streptococcus thermophilus
iodoacetate
-
 Streptococcus thermophilus
additional information enzyme is not influenced by high concentrations of aspartate up to 500 mM Streptococcus thermophilus
NEM
-
 Streptococcus thermophilus
p-chloromercuribenzoate
-
 Streptococcus thermophilus
thiol reagents
-
 Streptococcus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.7
-
 D-Asp
-
 Streptococcus thermophilus
35
-
 L-Asp
-
 Streptococcus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme contains no significant amount of metal Streptococcus thermophilus

Organism

Organism UniProt Comment Textmining
Streptococcus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus thermophilus

Reaction

Reaction Comment Organism Reaction ID
L-aspartate = D-aspartate removal of alpha-hydrogen of the substrate is at least partially rate-determining Streptococcus thermophilus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala no racemase activity Streptococcus thermophilus D-Ala
-
 ?
L-Asp
-
 Streptococcus thermophilus D-Asp
-
 ?
L-Cysteate at 88% of the activity relative to L-Asp Streptococcus thermophilus D-Cysteate
-
 ?
L-Cysteine sulfinate at 51% of the activity relative to L-Asp Streptococcus thermophilus D-Cysteine sulfinate
-
 ?
additional information no racemase activity with Asp, Cys, Pro, and hydroxyproline Streptococcus thermophilus ?
-
 ?