Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | removal of some divalent cation by EDTA gives slight increase in activity | Enterococcus faecalis | |
pyridoxal phosphate | racemase contains bound pyridoxal phosphate | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Enterococcus faecalis | |
Mn2+ | - |
Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
L-Asp | - |
Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
strain ATCC 9790, strain IAM 10065, no activity in strain IAM 10067 | - |
Purification (Comment) | Organism |
---|---|
- |
Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | at half the rate of Asp | Enterococcus faecalis | D-Ala | - |
? | |
L-Asp | - |
Enterococcus faecalis | D-Asp | - |
? | |
additional information | no racemase activity with Glu | Enterococcus faecalis | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
thermal stability shows a bimodal dependence on pH, with minimum stability at pH 7.5, and remarkably stability at pH 6 and pH 8-9. 10 min, pH 8, 66% loss of activity | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Enterococcus faecalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | thermal stability shows a bimodal dependence on pH, with minimum stability at pH 7.5, and remarkably stability at pH 6 and pH 8-9. 10 min, pH 8, 66% loss of activity | Enterococcus faecalis |