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Literature summary for 5.1.1.11 extracted from
- Biochemical evidence for conformational changes in the cross-talk between adenylation and peptidyl-carrier protein domains of nonribosomal peptide synthetases (2010), FEBS J., 277, 1159-1171.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed as a C-terminal His6-tag protein in Escherichia coli BL21 (DE3) | Brevibacillus brevis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C331A | minor changes in activity or specificity | Brevibacillus brevis |
| C331L | activity of GrsA is decreased to 26% | Brevibacillus brevis |
| C376S | no changes in activity | Brevibacillus brevis |
| C473A | no changes in activity | Brevibacillus brevis |
| C60F | no changes in activity | Brevibacillus brevis |
| C60F/C331A/C376S/C473A | named A-PCP(delta-4Cys), Km for L-Phe is increased by 2fold, while the kcat is reduced by 1.5fold | Brevibacillus brevis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0062 | - |
L-Phe | - |
Brevibacillus brevis |  |
| 0.0166 | - |
L-Phe | mutant A-PCP(delta-4Cys) | Brevibacillus brevis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73900 | - |
holo-A-PCP-form (GrsA plus peptidyl carrier protein domain), gel-filtration | Brevibacillus brevis |
| 74200 | - |
apo-A-PCP-form (GrsA plus peptidyl carrier protein domain), gel-filtration | Brevibacillus brevis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevibacillus brevis | P0C062 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Phe + H2O | - |
Brevibacillus brevis | AMP + diphosphate + D-Phe | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Gramicidin S synthetase I | - |
Brevibacillus brevis |
| GrsA | - |
Brevibacillus brevis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
L-Phe | mutant A-PCP(delta-4Cys) | Brevibacillus brevis | |
| 0.38 | - |
L-Phe | - |
Brevibacillus brevis | |
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Release 2023.1 (January 2023)
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