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Literature summary for 5.1.1.10 extracted from

  • Wang, L.; Ota, N.; Romanova, E.V.; Sweedler, J.V.
    A novel pyridoxal 5'-phosphate-dependent amino acid racemase in the Aplysia californica central nervous system (2011), J. Biol. Chem., 286, 13765-13774.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.1.10

Activating Compound

Activating Compound Comment Organism Structure
ATP ATP alone (4 mM) significantly increases enzyme activity, but when the combination of ATP and Mg2+ is present, the enzyme shows the largest activity for the D-Asp and D-Ser conversions Aplysia californica

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Aplysia californica

Inhibitors

Inhibitors Comment Organism Structure
aminooxyacetic acid complete inhibition at 0.002 mM Aplysia californica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8
-
 L-aspartate in 50 mM Tris-HCl buffer, pH 8.0, at 30°C Aplysia californica
15
-
 L-serine in 50 mM Tris-HCl buffer, pH 8.5, for Ser reactions, at 30°C Aplysia californica
16
-
 L-serine in 50 mM Tris-HCl buffer, pH 8.5, for Ser reactions, at 30°C Aplysia californica
94
-
 D-Aspartate in 50 mM Tris-HCl buffer, pH 8.0, at 30°C Aplysia californica

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgCl2 alone (2 mM) has only a small effect on enzyme racemase activity, but when Mg2+ and ATP are present, the enzyme shows the largest activity for the D-Asp and D-Ser conversions Aplysia californica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35400
-
 2 * 35400, calculated from amino acid sequence Aplysia californica
36000
-
 2 * 36000, SDS-PAGE Aplysia californica
57000
-
 gel filtration Aplysia californica

Organism

Organism UniProt Comment Textmining
Aplysia californica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sepharcryl 200 gel filtration Aplysia californica

Source Tissue

Source Tissue Comment Organism Textmining
atrial gland
-
 Aplysia californica
-
buccal muscle
-
 Aplysia californica
-
central nervous system DAR1 is localized to the medial region of the cerebral ganglion where the F- and C-clusters are situated Aplysia californica
-
liver
-
 Aplysia californica
-
mouth
-
 Aplysia californica
-
muscle
-
 Aplysia californica
-
ovotestis
-
 Aplysia californica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-aspartate lower substrate affinity for D-aspartate compared to L-aspartate Aplysia californica L-aspartate
-
 ?
D-serine similar substrate affinity for both L-serine and D-serine Aplysia californica L-serine
-
 ?
L-aspartate higher substrate affinity for L-aspartate compared to D-aspartate Aplysia californica D-aspartate
-
 ?
L-serine similar substrate affinity for both L-serine and D-serine Aplysia californica D-serine
-
 ?
additional information DAR1 has no detectable activity with 100 mM L-glutamate or 100 mM L-alanine Aplysia californica ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 36000, SDS-PAGE Aplysia californica
homodimer 2 * 35400, calculated from amino acid sequence Aplysia californica

Synonyms

Synonyms Comment Organism
D-amino acid racemase 1
-
 Aplysia californica
DAR1
-
 Aplysia californica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
14 50 the enzyme activity increases between 14-45°C and starts to decrease at 50°C for both the Ser and Asp conversion Aplysia californica

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5 the enzyme shows little racemase activity below pH 6.5 and an increasing activity between pH 7.0-8.5 in 50 mM Tris-HCl buffer Aplysia californica

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Aplysia californica