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Literature summary for 5.1.1.10 extracted from

  • Matsui, D.; Oikawa, T.; Arakawa, N.; Osumi, S.; Lausberg, F.; Staebler, N.; Freudl, R.; Eggeling, L.
    A periplasmic, pyridoxal-5-phosphate-dependent amino acid racemase in Pseudomonas taetrolens (2009), Appl. Microbiol. Biotechnol., 83, 1045-1054.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Pseudomonas taetrolens

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Pseudomonas taetrolens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41500
-
calculated from cDNA Pseudomonas taetrolens
84000
-
gel filtration Pseudomonas taetrolens

Organism

Organism UniProt Comment Textmining
Pseudomonas taetrolens I0J1I6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification N-terminal signal sequence is cleaved off Pseudomonas taetrolens

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Pseudomonas taetrolens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1120
-
recombinant protein Pseudomonas taetrolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala 12% activity, compared to D-Lys Pseudomonas taetrolens L-Ala
-
?
D-Arg 95% activity, compared to D-Lys Pseudomonas taetrolens L-Arg
-
?
D-ethionine 11% activity, compared to D-Lys Pseudomonas taetrolens L-ethionine
-
?
D-homoarginine 6.2% activity, compared to D-Lys Pseudomonas taetrolens L-homoarginine
-
?
D-Lys 100% activity Pseudomonas taetrolens L-Lys
-
?
D-Met 12% activity, compared to D-Lys Pseudomonas taetrolens L-Met
-
?
D-norvaline 6.5% activity, compared to D-Lys Pseudomonas taetrolens L-norvaline
-
?
D-ornithine 42% activity, compared to D-Lys Pseudomonas taetrolens L-ornithine
-
?

Subunits

Subunits Comment Organism
dimer 2 * 41500 Da Pseudomonas taetrolens

Synonyms

Synonyms Comment Organism
amino acid racemase
-
Pseudomonas taetrolens
ArgR
-
Pseudomonas taetrolens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pseudomonas taetrolens
65
-
maximum activity Pseudomonas taetrolens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
optimal activity Pseudomonas taetrolens
10
-
assay at Pseudomonas taetrolens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Pseudomonas taetrolens