Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.1.1 extracted from

  • Liu, D.; Liu, X.; Zhang, L.; Jiao, H.; Ju, J.; Zhao, B.
    Biochemical characteristics of an alanine racemase from Aeromonas hydrophila HBNUAh01 (2015), Microbiology, 84, 202-209 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene alr-2, sequence comparisons, the gene encodes a functional enzyme that can complement the alanine racemase deficiency of Escherichia coli strain MB2795, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Aeromonas hydrophila subsp. hydrophila

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ slightly inhibiting Aeromonas hydrophila subsp. hydrophila
Co2+ strongly inhibiting Aeromonas hydrophila subsp. hydrophila
DTT DTT at 1 mmol/l inhibits 67% of the enzyme activity compared with the control and DTT at 5 mmol/l results in complete inhibition of the enzyme activity Aeromonas hydrophila subsp. hydrophila
hydroxylamine via elimanting the cofactor pyridoxal-5'-phosphate from the enzyme Aeromonas hydrophila subsp. hydrophila
Mg2+ slightly inhibiting Aeromonas hydrophila subsp. hydrophila
additional information the enzyme activity is not sensitive to the metal chelating agent EDTA indicating that divalent cations are not required for enzyme activity Aeromonas hydrophila subsp. hydrophila
Na+ slightly inhibiting Aeromonas hydrophila subsp. hydrophila
Zn2+ strongly inhibiting Aeromonas hydrophila subsp. hydrophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15.36
-
D-alanine recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication Aeromonas hydrophila subsp. hydrophila
35.9
-
L-alanine recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication Aeromonas hydrophila subsp. hydrophila

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ enhances the catalytic activity up to 300% Aeromonas hydrophila subsp. hydrophila
additional information the enzyme activity is not sensitive to the metal chelating agent EDTA indicating that divalent cations are not required for enzyme activity Aeromonas hydrophila subsp. hydrophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine Aeromonas hydrophila subsp. hydrophila
-
D-alanine
-
r
L-alanine Aeromonas hydrophila subsp. hydrophila HBNUAh01
-
D-alanine
-
r

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila subsp. hydrophila A0KL98 strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240, very similar to strain HBNUAh01
-
Aeromonas hydrophila subsp. hydrophila HBNUAh01 A0KL98 strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240, very similar to strain HBNUAh01
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Aeromonas hydrophila subsp. hydrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine
-
Aeromonas hydrophila subsp. hydrophila D-alanine
-
r
L-alanine
-
Aeromonas hydrophila subsp. hydrophila HBNUAh01 D-alanine
-
r

Subunits

Subunits Comment Organism
? x * 40390, sequence calculation, x * 43000, recombinant His-tagged enzyme, SDS-PAGE Aeromonas hydrophila subsp. hydrophila

Synonyms

Synonyms Comment Organism
alr-2
-
Aeromonas hydrophila subsp. hydrophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
50 purified recombinant enzyme, stable up to, over 90% activity remaining Aeromonas hydrophila subsp. hydrophila
60
-
purified recombinant enzyme, inactivation Aeromonas hydrophila subsp. hydrophila

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 11 purified recombinant enzyme, stable at, over 80% activity remaining Aeromonas hydrophila subsp. hydrophila

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate PLP, dependent on, no catalytic enzyme activity without, the apoenzyme activity is completely inhibited Aeromonas hydrophila subsp. hydrophila