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Literature summary for 5.1.1.1 extracted from

  • Wang, Y.; Yang, C.; Xue, W.; Zhang, T.; Liu, X.; Ju, J.; Zhao, B.; Liu, D.
    Selection and characterization of alanine racemase inhibitors against Aeromonas hydrophila (2017), BMC Microbiol., 17, 122-138 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene alr-2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Aeromonas hydrophila subsp. hydrophila

Inhibitors

Inhibitors Comment Organism Structure
2-(9-acridinylamino)benzenepropanoic acid
-
 Aeromonas hydrophila subsp. hydrophila
anabellamide
-
 Aeromonas hydrophila subsp. hydrophila
D-cycloserine
-
 Aeromonas hydrophila subsp. hydrophila
hematoxylin
-
 Aeromonas hydrophila subsp. hydrophila
higenamine
-
 Aeromonas hydrophila subsp. hydrophila
homogentisic acid competitive inhibition, has minimal cytotoxicity against mammalian cells. Homogentisic acid binds to the active site of the racemase Aeromonas hydrophila subsp. hydrophila
hydroquinone noncompetitive inhibition, has minimal cytotoxicity against mammalian cells. Hydroquinone binds near the active center of alanine racemase Aeromonas hydrophila subsp. hydrophila
additional information inhibitor screening of a library of 2100 compounds, and molecular docking study Aeromonas hydrophila subsp. hydrophila
patulin
-
 Aeromonas hydrophila subsp. hydrophila
propyl gallate
-
 Aeromonas hydrophila subsp. hydrophila
quercetin
-
 Aeromonas hydrophila subsp. hydrophila
[4-(5-butyl-5-methyl-2(5H)-furanylidene)dihydro-3,5-dioxo-2(3H)-furanylidene]acetic acid
-
 Aeromonas hydrophila subsp. hydrophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine Aeromonas hydrophila subsp. hydrophila
-
 D-alanine
-
 r
L-alanine Aeromonas hydrophila subsp. hydrophila HBNUAh01 / ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240
-
 D-alanine
-
 r

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila subsp. hydrophila A0KL98
-
-
Aeromonas hydrophila subsp. hydrophila HBNUAh01 / ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240 A0KL98
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Aeromonas hydrophila subsp. hydrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine
-
 Aeromonas hydrophila subsp. hydrophila D-alanine
-
 r
L-alanine
-
 Aeromonas hydrophila subsp. hydrophila HBNUAh01 / ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240 D-alanine
-
 r
additional information assay method optimization and validation, overview Aeromonas hydrophila subsp. hydrophila ?
-
 ?
additional information assay method optimization and validation, overview Aeromonas hydrophila subsp. hydrophila HBNUAh01 / ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Aeromonas hydrophila subsp. hydrophila

Synonyms

Synonyms Comment Organism
alr-2
-
 Aeromonas hydrophila subsp. hydrophila
alr-2 gen ename Aeromonas hydrophila subsp. hydrophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Aeromonas hydrophila subsp. hydrophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
 Aeromonas hydrophila subsp. hydrophila

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Aeromonas hydrophila subsp. hydrophila

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0517
-
 homogentisic acid pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila
0.212
-
 hydroquinone pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0054
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila D-cycloserine
0.0066
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila anabellamide
0.0086
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila propyl gallate
0.0089
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila [4-(5-butyl-5-methyl-2(5H)-furanylidene)dihydro-3,5-dioxo-2(3H)-furanylidene]acetic acid
0.0125
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila homogentisic acid
0.0143
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila higenamine
0.0147
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila patulin
0.0155
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila quercetin
0.0156
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila hematoxylin
0.0177
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila 2-(9-acridinylamino)benzenepropanoic acid
0.0185
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila hydroquinone

General Information

General Information Comment Organism
additional information enzyme structure homology modeling using the crystal structure of Escherichia coli AlaR with PDB ID 2rjh Aeromonas hydrophila subsp. hydrophila
physiological function the enzyme catalyzes the interconversion of L-alanine to D-alanine. D-Alanine is an essential building block of the cell wall of both gram-positive and gram-negative bacteria Aeromonas hydrophila subsp. hydrophila