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Literature summary for 5.1.1.1 extracted from

  • Dong, H.; Xu, S.; Lu, X.; He, G.; Zhao, R.; Chen, S.; Fu, S.; Ju, J.
    Crystallization and preliminary X-ray study of a thermostable alanine racemase from Thermoanaerobacter tengcongensis MB4 (2013), Acta Crystallogr. Sect. F, 69, 660-662.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene alrMB4, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Caldanaerobacter subterraneus subsp. tengcongensis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 12 mg/ml protein in 10 mM Tris-HCl, pH 8.0 with 10 mM pyridoxal 5'-phosphate, with 0.1 M bis-tris pH 7.0, 22% w/v PEG 4000, X-ray diffraction structure determination and analysis at 2.6 A resolution Caldanaerobacter subterraneus subsp. tengcongensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 47000, recombinant His6-tagged enzyme, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine Caldanaerobacter subterraneus subsp. tengcongensis
-
 D-alanine
-
 r

Organism

Organism UniProt Comment Textmining
Caldanaerobacter subterraneus subsp. tengcongensis Q8RAK6 gene alrMB4
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration to apparent homogeneity Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine
-
 Caldanaerobacter subterraneus subsp. tengcongensis D-alanine
-
 r

Subunits

Subunits Comment Organism
? x * 47000, recombinant His6-tagged enzyme, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
More three-dimensional enzyme structure of the recombinnat His6-tagged enzyme, overview Caldanaerobacter subterraneus subsp. tengcongensis

Synonyms

Synonyms Comment Organism
ALR
-
 Caldanaerobacter subterraneus subsp. tengcongensis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Caldanaerobacter subterraneus subsp. tengcongensis