Cloned (Comment) | Organism |
---|---|
enzyme expression in Escherichia coli strain BL21(DE3)/pMB1978 | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme from strain Mu50, sitting drop method, mixing of 12 mg/ml protein in 20 mM Tris-HCl, pH 7.6, with 100 mM sodium acetate trihydrate, pH 5.0, and 1.8 M ammonium sulfate, 7 days, X-ray diffraction structure determination and analysis at 2.15 A resolution, molecular replacement | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the active-site binding pocket, dimer interface and active-site entryway of the enzyme are potential targets for structure-aided inhibitor design, formation of a template for structure-based drug-development efforts targeting the enzyme, overview | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.89 | - |
D-alanine | pH not specified in the publication, 30°C, recombinant enzyme | Staphylococcus aureus | |
2.77 | - |
L-alanine | pH not specified in the publication, 30°C, recombinant enzyme | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42800 | - |
2 * 42800, recombinant enzyme, SDS-PAGE | Staphylococcus aureus |
73000 | - |
recombinant enzyme, dynamic light scattering | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | Staphylococcus aureus | - |
D-alanine | - |
r | |
L-alanine | Staphylococcus aureus Mu50 | - |
D-alanine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | P63479 | - |
- |
Staphylococcus aureus Mu50 | P63479 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3)/pMB1978 by anion exchange and hydrophobic interaction chromatography, followed by gel filtration and dialysis to over 95% purity | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | - |
Staphylococcus aureus | D-alanine | - |
r | |
L-alanine | - |
Staphylococcus aureus Mu50 | D-alanine | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42800, recombinant enzyme, SDS-PAGE | Staphylococcus aureus |
More | dimer interfaces for the enzyme is 2510 A2 | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
ALR | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
malfunction | because D-alanine is an essential component of the bacterial cell-wall peptidoglycan, inhibition of alanine racemase is lethal to prokaryotes | Staphylococcus aureus |