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Literature summary for 5.1.1.1 extracted from

  • Pierce, K.J.; Salifu, S.P.; Tangney, M.
    Gene cloning and characterization of a second alanine racemase from Bacillus subtilis encoded by yncD (2009), FEMS Microbiol. Lett., 283, 69-74.
    View publication on PubMed
Search for more literature for 5.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in D-Alanine auxotrophic Escherichia coli strain MB2159 and DN1686, a D-Ala auxotrophic mutant Bacillus subtilis
recombinant expression in D-Alanine auxotrophic Escherichia coli strains MB2159 and DN1686, a D-Ala auxotrophic mutant Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43650
-
 precided weight from primary sequence of 394 amino acids, yncD Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine Bacillus subtilis
-
 D-alanine
-
 r
L-alanine Bacillus subtilis 168
-
 D-alanine
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P10725
-
-
Bacillus subtilis P94494
-
-
Bacillus subtilis 168 P10725
-
-
Bacillus subtilis 168 P94494
-
-
no activity in Bacillus subtilis
-
 D-Ala auxotrophic mutant
-
no activity in Bacillus subtilis DN1686
-
 D-Ala auxotrophic mutant
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine
-
 Bacillus subtilis D-alanine
-
 r
L-alanine
-
 Bacillus subtilis 168 D-alanine
-
 r

Synonyms

Synonyms Comment Organism
alanine racemase
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 dal, extracts are assayed (in triplicate) by monitoring NADH production in spectrophotometric assay with L-alanine dehydrogenase Bacillus subtilis
55
-
 yncD, extracts are assayed (in triplicate) by monitoring NADH production in spectrophotometric assay with L-alanine dehydrogenase Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: approx. 25% of max. activity, 70°C: approx. 8% of max. activity, yncD, extracts are assayed (in triplicate) by monitoring NADH production in spectrophotometric assay with L-alanine dehydrogenase Bacillus subtilis
30 60 30°C and 60°C: approx. 20% of max. activity, dal, extracts are assayed (in triplicate) by monitoring NADH production in spectrophotometric assay with L-alanine dehydrogenase Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 dal, extracts are assayed (in triplicate) by monitoring NADH production in spectrophotometric assay with L-alanine dehydrogenase Bacillus subtilis
10 10.5 yncD, extracts are assayed (in triplicate) by monitoring NADH production in spectrophotometric assay with L-alanine dehydrogenase Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
8.5 11 pH 8.5: 35% of max. activity, pH 11: 8% of max. activity, dal, extracts are assayed (in triplicate) by monitoring NADH production in spectrophotometric assay with L-alanine dehydrogenase Bacillus subtilis
8.5 11 pH 8.5: 42% of max. activity, pH 11: 58% of max. activity, yncD, extracts are assayed (in triplicate) by monitoring NADH production in spectrophotometric assay with L-alanine dehydrogenase Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Bacillus subtilis