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Literature summary for 5.1.1.1 extracted from

  • Cassimjee, K.E.; Trummer, M.; Branneby, C.; Berglund, P.
    Silica-immobilized His(6)-tagged enzyme: Alanine racemase in hydrophobic solvent (2007), Biotechnol. Bioeng., 99 (3), 712-716.
    View publication on PubMed
Search for more literature for 5.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 enzyme including linker and His6-tag Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine Geobacillus stearothermophilus The bacterium utilizes D-alanine (DAla) for synthesis of the peptidoglycan cell wall. D-alanine
-
 r

Organic Solvent Stability

Organic Solvent Comment Organism
hexane no significant change in activity Geobacillus stearothermophilus
additional information ethyl acetate destroyes the enzyme activity Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
 ATCC 7953
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine The bacterium utilizes D-alanine (DAla) for synthesis of the peptidoglycan cell wall. Geobacillus stearothermophilus D-alanine
-
 r

Synonyms

Synonyms Comment Organism
alanine racemase
-
 Geobacillus stearothermophilus
AlaR
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 thermostable Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Geobacillus stearothermophilus