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Literature summary for 5.1.1.1 extracted from

  • Noda, M.; Kawahara, Y.; Ichikawa, A.; Matoba, Y.; Matsuo, H.; Lee, D.G.; Kumagai, T.; Sugiyama, M.
    Self-protection mechanism in D-cycloserine-producing Streptomyces lavendulae. Gene cloning, characterization, and kinetics of its alanine racemase and D-alanyl-D-alanine ligase, which are target enzymes of D-cycloserine (2004), J. Biol. Chem., 279, 46143-46152.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli
-
Streptomyces lavendulae

Inhibitors

Inhibitors Comment Organism Structure
D-cycloserine time-dependent inactivation rate of enzyme from Streptomyces lavendulae is slower than for enzyme from Escherichia coli Escherichia coli
D-cycloserine time-dependent inactivation rate of enzyme from Streptomyces lavendulae is slower than for enzyme from Escherichia coli. Enzyme from Streptomyces lavendulae is one of its self-resistance determinants Streptomyces lavendulae
L-Cycloserine
-
Escherichia coli
additional information not inhibitory: L-cycloserine Streptomyces lavendulae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
L-alanine pH 8.2, 25°C Streptomyces lavendulae
0.7
-
D-alanine pH 8.2, 25°C Streptomyces lavendulae

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Streptomyces lavendulae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39900
-
2 * 39900, calculated, 2 * 42000, SDs-PAGE Escherichia coli
39900
-
2 * 39900, calculated, 2 * 42000, SDs-PAGE Streptomyces lavendulae
42000
-
2 * 39900, calculated, 2 * 42000, SDs-PAGE Escherichia coli
42000
-
2 * 39900, calculated, 2 * 42000, SDs-PAGE Streptomyces lavendulae
80000
-
gel filtration Escherichia coli
80000
-
gel filtration Streptomyces lavendulae

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Streptomyces lavendulae Q65YW7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Escherichia coli
recombinant protein Streptomyces lavendulae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine
-
Escherichia coli L-alanine
-
r
D-alanine
-
Streptomyces lavendulae L-alanine
-
r
L-alanine
-
Escherichia coli D-alanine
-
r
L-alanine
-
Streptomyces lavendulae D-alanine
-
r

Subunits

Subunits Comment Organism
dimer 2 * 39900, calculated, 2 * 42000, SDs-PAGE Escherichia coli
dimer 2 * 39900, calculated, 2 * 42000, SDs-PAGE Streptomyces lavendulae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
55
-
L-alanine pH 8.2, 25°C Streptomyces lavendulae
70
-
D-alanine pH 8.2, 25°C Streptomyces lavendulae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.87
-
D-cycloserine pH 8.2, 25°C Streptomyces lavendulae
1.2
-
D-cycloserine pH 8.2, 25°C Escherichia coli
8.2
-
L-Cycloserine pH 8.2, 25°C Escherichia coli