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Literature summary for 5.1.1.1 extracted from
- High catalytic activity of alanine racemase from psychrophilic Bacillus psychrosaccharolyticus at high temperatures in the presence of pyridoxal 5'-phosphate (2000), FEMS Microbiol. Lett., 192, 169-173.
General Stability
| General Stability | Organism |
|---|---|
| pyridoxal 5'-phosphate present in the solvent suppresses the enzyme's unfolding | Peribacillus psychrosaccharolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Peribacillus psychrosaccharolyticus | - |
- |
- |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Peribacillus psychrosaccharolyticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | 40°C: about 60% of maximal activity, 70°C: about 50% of maximal activity | Peribacillus psychrosaccharolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
the decrease in the enzyme activity at incubation temperatures over 40°C is consistent with the decrease in the amount of bound pyridoxal 5'-phosphate, no effect on stability at lower temperatures, 0-30°C | Peribacillus psychrosaccharolyticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | Km at 30°C is 0.005 mM. Maximal activity is obtained in presence of more than 0.125 mM pyridoxal 5'-phosphate. The decrease in activity at incubation temperatures over 40°C is consistent with the decrease in the amount of bound pyridoxal 5'-phosphate | Peribacillus psychrosaccharolyticus |  |
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