Crystallization (Comment) | Organism |
---|---|
hanging drop method using purified enzyme concentrated to 22 mg/ml. The hanging drop contains 0.01 ml of the protein solution, 0.01 ml of 23% polyethylene glycol 4K, 200 mM sodium acetate and 100 mM Tris, pH 8.5. Drops are equilibrated against 0.7 ml of polyethylene glycol 4K solution | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cycloserine | suicide inhibitor | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | Geobacillus stearothermophilus | the enzyme catalyzes the first committed step in bacterial cell wall biosynthesis | D-Ala | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P10724 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | the enzyme catalyzes the first committed step in bacterial cell wall biosynthesis | Geobacillus stearothermophilus | D-Ala | - |
? |