Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | inactivates the enzyme at high concentrations | Arabidopsis thaliana | |
Fe2+ | inactivates the enzyme at high concentrations | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | it is not possible to measure an accurate KM value for the metals as at low concentration of metal the assay is not sensitive enough, whereas at higher metal ion concentrations the enzyme is inactivated | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | inactivates the enzyme at high concentrations | Arabidopsis thaliana | |
Fe2+ | inactivates the enzyme at high concentrations | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Fe2+ | Arabidopsis thaliana | - |
siroheme + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0054 | - |
isolated recombinant enzyme, with ferrous iron as substrate | Arabidopsis thaliana |
0.0485 | - |
isolated recombinant enzyme, with cobalt as substrate | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Co2+ | pH 8.0 | Arabidopsis thaliana | cobalt-sirohydrochlorin + H+ | - |
? | |
sirohydrochlorin + Fe2+ | - |
Arabidopsis thaliana | siroheme + H+ | - |
? | |
sirohydrochlorin + Fe2+ | pH 8.0 | Arabidopsis thaliana | siroheme + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sirohydrochlorin ferrochelatase | - |
Arabidopsis thaliana |