Cloned (Comment) | Organism |
---|---|
- |
Salmonella enterica |
Crystallization (Comment) | Organism |
---|---|
X-ray crystal structure of CysG | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
S128A | mutant has higher cobalt chelatase activity than wild-type CysG with sirohydrochlorin as substrate | Salmonella enterica |
S128D | mutant has lower cobalt chelatase activity than wild-type CysG with sirohydrochlorin as substrate | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Fe2+ | Salmonella enterica | CysG, siroheme biosynthesis | siroheme + 2 H+ | sulfur metabolism depends on siroheme | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | CysG, phosphorylation of Ser-128, plays a regulatory role | Salmonella enterica |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0138 | - |
S128D mutant CysG, cobalt chelation of sirohydrochlorin | Salmonella enterica |
0.0656 | - |
wild-type CysG, cobalt chelation of sirohydrochlorin | Salmonella enterica |
0.243 | - |
S128A mutant CysG, cobalt chelation of sirohydrochlorin | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Co2+ | - |
Salmonella enterica | cobalt-sirohydrochlorin + 2 H+ | - |
? | |
sirohydrochlorin + Fe2+ | CysG structure, the multifunctional siroheme synthase CysG synthesizes siroheme from uroporphyrinogen III, CysG contains two structurally independent modules: a bismethyltransferase and a dual-function dehydrogenase-chelatase | Salmonella enterica | siroheme + 2 H+ | - |
? | |
sirohydrochlorin + Fe2+ | CysG, siroheme biosynthesis | Salmonella enterica | siroheme + 2 H+ | sulfur metabolism depends on siroheme | ? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Salmonella enterica |
Synonyms | Comment | Organism |
---|---|---|
CysG | Salmonella enterica | Salmonella enterica |