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Literature summary for 4.99.1.4 extracted from

  • Raux, E.; Leech, H.K.; Beck, R.; Schubert, H.L.; Santander, P.J.; Roessner, C.A.; Scott, A.I.; Martens, J.H.; Jahn, D.; Thermes, C.; Rambach, A.; Warren, M.J.
    Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium (2003), Biochem. J., 370, 505-516.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.99.1.4

Cloned(Commentary)

Cloned (Comment) Organism
sirB, expression in Escherichia coli BL21(DE3)pLysS, sirA,B,C operon, amino acid sequence Priestia megaterium

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble recombinant SirB Priestia megaterium
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 1 * 33000, SDS-PAGE Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sirohydrochlorin + Fe2+ Priestia megaterium SirB is responsible for the final step in siroheme synthesis siroheme + 2 H+
-
 ?
sirohydrochlorin + Fe2+ Priestia megaterium DSM 509 SirB is responsible for the final step in siroheme synthesis siroheme + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
 strain DSM509
-
Priestia megaterium DSM 509
-
 strain DSM509
-

Purification (Commentary)

Purification (Comment) Organism
recombinant SirB Priestia megaterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0034
-
 pH 8, cobaltochelation of precorrin-2 Priestia megaterium
0.337
-
 pH 8, cobaltochelation of sirohydrochlorin Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
precorrin-2 + Co2+ SirB, much lower specific activity than with sirohydrochlorin Priestia megaterium cobalt-precorrin-2 + 2 H+
-
 ?
precorrin-2 + Co2+ SirB, much lower specific activity than with sirohydrochlorin Priestia megaterium DSM 509 cobalt-precorrin-2 + 2 H+
-
 ?
sirohydrochlorin + Co2+ SirB, lower specificity for cobalt than for iron Priestia megaterium cobalt-sirohydrochlorin + 2 H+
-
 ?
sirohydrochlorin + Co2+ SirB, lower specificity for cobalt than for iron Priestia megaterium DSM 509 cobalt-sirohydrochlorin + 2 H+
-
 ?
sirohydrochlorin + Fe2+ SirB, monofunctional ferrochelatase, higher specificity for iron over cobalt Priestia megaterium siroheme + 2 H+
-
 ?
sirohydrochlorin + Fe2+ SirB is responsible for the final step in siroheme synthesis Priestia megaterium siroheme + 2 H+
-
 ?
sirohydrochlorin + Fe2+ SirB, monofunctional ferrochelatase, higher specificity for iron over cobalt Priestia megaterium DSM 509 siroheme + 2 H+
-
 ?
sirohydrochlorin + Fe2+ SirB is responsible for the final step in siroheme synthesis Priestia megaterium DSM 509 siroheme + 2 H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Priestia megaterium

Synonyms

Synonyms Comment Organism
SirB Bacillus megaterium Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Priestia megaterium