Cloned (Comment) | Organism |
---|---|
MET8 gene | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
X-ray crystal structure of Met8p, hanging drop method | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D141A | mutant of bifunctional Met8p is completely inactive as both dehydrogenase and ferrochelatase | Saccharomyces cerevisiae |
G22D | mutant of bifunctional Met8p is completely inactive as dehydrogenase, but functions as ferrochelatase | Saccharomyces cerevisiae |
H237A | mutant of bifunctional Met8p is active as both dehydrogenase and ferrochelatase | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Fe2+ | Saccharomyces cerevisiae | - |
siroheme + H+ | - |
? | |
sirohydrochlorin + Fe2+ | Saccharomyces cerevisiae | Met8p catalyzes the final two steps in the biosynthesis of siroheme involving a beta-NAD+-dependent dehydrogenation of precorrin-2 to generate sirohydrochlorin followed by ferrochelation to yield siroheme | siroheme + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae | P15807 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant Met8p | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
0.0311 | - |
wild-type Met8p | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Co2+ | - |
Saccharomyces cerevisiae | cobalt-sirohydrochlorin + 2 H+ | - |
? | |
sirohydrochlorin + Fe2+ | - |
Saccharomyces cerevisiae | siroheme + H+ | - |
? | |
sirohydrochlorin + Fe2+ | Met8p structure, bifunctional Met8p catalyzes the final two steps in the biosynthesis of siroheme involving a beta-NAD+-dependent dehydrogenation of precorrin-2 to generate sirohydrochlorin followed by ferrochelation to yield siroheme, both catalytic activities share a single active site, Asp-141 functions as a general base and plays an essential role in both dehydrogenase and chelatase processes | Saccharomyces cerevisiae | siroheme + 2 H+ | - |
? | |
sirohydrochlorin + Fe2+ | Met8p catalyzes the final two steps in the biosynthesis of siroheme involving a beta-NAD+-dependent dehydrogenation of precorrin-2 to generate sirohydrochlorin followed by ferrochelation to yield siroheme | Saccharomyces cerevisiae | siroheme + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | three structural domains per monomer, domain structure | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Met8p | Saccharomyces cerevisiae | Saccharomyces cerevisiae |
More | Met8p is a class 2 chelatase | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharomyces cerevisiae |