BRENDA - Enzyme Database show
show all sequences of 4.99.1.3

Evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization

Romao, C.V.; Ladakis, D.; Lobo, S.A.; Carrondo, M.A.; Brindley, A.A.; Deery, E.; Matias, P.M.; Pickersgill, R.W.; Saraiva, L.M.; Warren, M.J.; Proc. Natl. Acad. Sci. USA 108, 97-102 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Desulfovibrio vulgaris
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 100 mM Tris-HCl. pH 8.5, containing 2.0 M ammonium sulfate
Desulfovibrio vulgaris
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Archaeoglobus fulgidus
-
-
periplasm
-
Desulfovibrio vulgaris
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Archaeoglobus fulgidus
-
-
-
Salmonella enterica
-
-
-
Desulfovibrio vulgaris
Q72EC8
-
-
Purification (Commentary)
Commentary
Organism
-
Desulfovibrio vulgaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sirohydrochlorin + Co2+
-
716791
Salmonella enterica
cobalt-sirohydrochlorin + H+
-
-
-
?
sirohydrochlorin + Co2+
-
716791
Archaeoglobus fulgidus
cobalt-sirohydrochlorin + H+
-
-
-
?
sirohydrochlorin + Co2+
-
716791
Desulfovibrio vulgaris
cobalt-sirohydrochlorin + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
-
Archaeoglobus fulgidus
tetramer
x-ray crystallography
Desulfovibrio vulgaris
tetramer
-
Salmonella enterica
Cofactor
Cofactor
Commentary
Organism
Structure
heme
heme b
Archaeoglobus fulgidus
heme
heme b
Desulfovibrio vulgaris
heme
heme b
Salmonella enterica
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Desulfovibrio vulgaris
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
heme b
Archaeoglobus fulgidus
heme
heme b
Desulfovibrio vulgaris
heme
heme b
Salmonella enterica
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 100 mM Tris-HCl. pH 8.5, containing 2.0 M ammonium sulfate
Desulfovibrio vulgaris
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Archaeoglobus fulgidus
-
-
periplasm
-
Desulfovibrio vulgaris
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Desulfovibrio vulgaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sirohydrochlorin + Co2+
-
716791
Salmonella enterica
cobalt-sirohydrochlorin + H+
-
-
-
?
sirohydrochlorin + Co2+
-
716791
Archaeoglobus fulgidus
cobalt-sirohydrochlorin + H+
-
-
-
?
sirohydrochlorin + Co2+
-
716791
Desulfovibrio vulgaris
cobalt-sirohydrochlorin + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
-
Archaeoglobus fulgidus
tetramer
x-ray crystallography
Desulfovibrio vulgaris
tetramer
-
Salmonella enterica
Other publictions for EC 4.99.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747658
Lobo
Desulfovibrio vulgaris CbiKP ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris DSM 644
Environ. Microbiol.
19
106-118
2017
-
-
1
1
7
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
7
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730419
Bali
Identification and characteriz ...
Paracoccus denitrificans, Paracoccus pantotrophus
Mol. Microbiol.
92
153-163
2014
-
-
1
-
2
-
-
-
-
-
1
2
-
4
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730479
Moore
Towards a cell factory for vit ...
Bacillus megaterium, Bacillus megaterium DSM 319
New Biotechnol.
31
553-561
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
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2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
716791
Romao
Evolution in a family of chela ...
Archaeoglobus fulgidus, Desulfovibrio vulgaris, Salmonella enterica
Proc. Natl. Acad. Sci. USA
108
97-102
2011
-
-
1
1
-
-
-
-
2
-
-
-
-
3
-
-
1
-
-
-
-
-
3
3
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
1
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699853
Lundqvist
The AAA(+) motor complex of su ...
Brucella melitensis
J. Struct. Biol.
167
227-234
2009
-
-
1
-
-
-
-
-
-
-
3
1
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690966
Lobo
Two distinct roles for two fun ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris Hildenborough
Biochemistry
47
5851-5857
2008
-
-
1
-
1
-
-
-
2
-
2
10
-
4
-
2
1
-
-
-
6
-
24
1
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
2
-
-
-
-
-
2
-
2
10
-
-
2
2
-
-
6
-
24
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682635
Pisarchik
Probing the structural plastic ...
Methanosarcina barkeri
Protein Eng. Des. Sel.
20
257-265
2007
-
-
1
1
3
-
-
2
-
-
11
-
-
2
-
-
1
-
-
-
11
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
3
-
-
-
-
2
-
-
11
-
-
-
-
1
-
-
11
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681658
Yin
Crystal structure of the vitam ...
Archaeoglobus fulgidus
J. Struct. Funct. Genomics
7
37-50
2006
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644512
Brindley
A story of chelatase evolution ...
Methanobacter thermoautotrophicum, Methanosarcina barkeri, Methanosarcina barkeri Fusaro / DSM 804
J. Biol. Chem.
278
22388-22395
2003
-
-
2
-
-
-
-
-
-
5
6
6
-
17
-
-
2
-
-
-
3
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
5
6
6
-
-
-
2
-
-
3
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
652468
Leech
Characterization of the cobalt ...
Bacillus megaterium, Bacillus megaterium DSM 509, Synechocystis sp.
J. Biol. Chem.
278
41900-41907
2003
-
-
2
-
3
-
-
-
-
2
2
3
-
10
-
-
2
-
-
-
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
2
2
3
-
-
-
2
-
-
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649817
Schubert
Structural diversity in metal ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochem. Soc. Trans.
30
595-600
2002
-
-
-
1
-
-
-
-
-
1
-
1
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649818
Leech
Production of cobalamin and si ...
Bacillus megaterium
Biochem. Soc. Trans.
30
610-613
2002
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650801
Raux
Biosynthesis of cobalamin (vit ...
Bacillus megaterium, Clostridioides difficile, Clostridium acetobutylicum, no activity in Bacillus subtilis, no activity in eukaryota, Porphyromonas gingivalis, Pseudomonas aeruginosa, Rhodobacter sphaeroides, Salmonella enterica subsp. enterica serovar Typhimurium
Cell. Mol. Life Sci.
57
1880-1893
2000
-
-
1
1
-
-
-
-
-
-
-
7
-
10
1
-
1
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
7
-
1
-
1
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644511
Schubert
Common chelatase design in the ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochemistry
38
10660-10669
1999
-
-
1
1
1
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
3
-
-
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-
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-
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-
1
-
1
1
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-
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-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651631
Raux
A role for Salmonella typhimur ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
179
3202-3212
1997
-
-
1
-
-
-
-
-
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1
-
4
-
-
-
-
-
-
-
-
2
-
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1
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1
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2
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