BRENDA - Reference to 4.99.1.3; Id = 699853

genes cobN, cobS and cobT, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3)

Brucella melitensis

37000

-

x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT

Brucella melitensis

71000

-

x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT

Brucella melitensis

137000

-

x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT

Brucella melitensis

sirohydrochlorin + Co2+

Brucella melitensis

involved in the formation of a octahedrally co-ordinated cobalt ion. In the oxygen-dependent cobalamin, i.e. vitamin B12, biosynthetic pathway, cobalt is inserted into a ring-contracted tetrapyrrole called hydrogenobyrinic acid a,c-diamide, HBAD, by a cobaltochelatase that is constituted by three subunits, CobN, CobS and CobT

cobalt-sirohydrochlorin + 2 H+

-

?

Brucella melitensis

-

genes cobN, cobS and cobT encoding the tree subunits

-

sirohydrochlorin + Co2+

involved in the formation of a octahedrally co-ordinated cobalt ion. In the oxygen-dependent cobalamin, i.e. vitamin B12, biosynthetic pathway, cobalt is inserted into a ring-contracted tetrapyrrole called hydrogenobyrinic acid a,c-diamide, HBAD, by a cobaltochelatase that is constituted by three subunits, CobN, CobS and CobT

699853

Brucella melitensis

cobalt-sirohydrochlorin + 2 H+

-

?

More

the molecules are arranged in a two-tiered ring structure with the six subunits in each ring organized as a trimer of dimers, subunits CobS and CobT form a chaperone-like complex. Homology modelling of subunit CobS, overview

Brucella melitensis

oligomer

x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT

Brucella melitensis

genes cobN, cobS and cobT, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3)

Brucella melitensis

37000

-

x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT

Brucella melitensis

71000

-

x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT

Brucella melitensis

137000

-

x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT

Brucella melitensis

sirohydrochlorin + Co2+

Brucella melitensis

involved in the formation of a octahedrally co-ordinated cobalt ion. In the oxygen-dependent cobalamin, i.e. vitamin B12, biosynthetic pathway, cobalt is inserted into a ring-contracted tetrapyrrole called hydrogenobyrinic acid a,c-diamide, HBAD, by a cobaltochelatase that is constituted by three subunits, CobN, CobS and CobT

cobalt-sirohydrochlorin + 2 H+

-

?

sirohydrochlorin + Co2+

involved in the formation of a octahedrally co-ordinated cobalt ion. In the oxygen-dependent cobalamin, i.e. vitamin B12, biosynthetic pathway, cobalt is inserted into a ring-contracted tetrapyrrole called hydrogenobyrinic acid a,c-diamide, HBAD, by a cobaltochelatase that is constituted by three subunits, CobN, CobS and CobT

699853

Brucella melitensis

cobalt-sirohydrochlorin + 2 H+

-

?

More

the molecules are arranged in a two-tiered ring structure with the six subunits in each ring organized as a trimer of dimers, subunits CobS and CobT form a chaperone-like complex. Homology modelling of subunit CobS, overview

Brucella melitensis

oligomer

x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT

Brucella melitensis

747658

Lobo

Desulfovibrio vulgaris CbiKP ...

Desulfovibrio vulgaris, Desulfovibrio vulgaris DSM 644

Environ. Microbiol.

19

106-118

2017

-

1

7

-

1

-

2

-

1

-

1

-

1

7

-

1

-

1

-

1

-

730419

Bali

Identification and characteriz ...

Paracoccus denitrificans, Paracoccus pantotrophus

Mol. Microbiol.

92

153-163

2014

-

1

-

2

-

1

2

-

4

-

1

-

2

1

-

1

-

2

-

1

2

-

1

-

2

1

-

1

-

730479

Moore

Towards a cell factory for vit ...

Bacillus megaterium, Bacillus megaterium DSM 319

New Biotechnol.

31

553-561

2014

-

2

-

4

-

2

-

2

-

2

-

1

-

1

-

716791

Romao

Evolution in a family of chela ...

Archaeoglobus fulgidus, Desulfovibrio vulgaris, Salmonella enterica

Proc. Natl. Acad. Sci. USA

108

97-102

2011

-

1

-

2

-

3

-

1

-

3

-

3

-

1

3

1

-

2

-

1

-

3

-

699853

Lundqvist

The AAA(+) motor complex of su ...

Brucella melitensis

J. Struct. Biol.

167

227-234

2009

-

1

-

3

1

-

1

-

1

2

-

1

-

3

1

-

1

2

-

690966

Lobo

Two distinct roles for two fun ...

Desulfovibrio vulgaris, Desulfovibrio vulgaris Hildenborough

Biochemistry

47

5851-5857

2008

-

1

-

1

-

2

-

2

10

-

4

-

2

1

-

6

-

24

1

-

1

-

2

1

-

2

-

2

-

2

10

-

2

-

6

-

24

2

-

682635

Pisarchik

Probing the structural plastic ...

Methanosarcina barkeri

Protein Eng. Des. Sel.

20

257-265

2007

-

1

3

-

2

-

11

-

2

-

1

-

11

-

1

-

1

-

1

3

-

2

-

11

-

1

-

11

-

1

-

681658

Yin

Crystal structure of the vitam ...

Archaeoglobus fulgidus

J. Struct. Funct. Genomics

7

37-50

2006

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

2

-

1

-

644512

Brindley

A story of chelatase evolution ...

Methanobacter thermoautotrophicum, Methanosarcina barkeri, Methanosarcina barkeri Fusaro / DSM 804

J. Biol. Chem.

278

22388-22395

2003

-

2

-

5

6

-

17

-

2

-

3

-

12

-

2

-

5

6

-

2

-

3

-

12

-

652468

Leech

Characterization of the cobalt ...

Bacillus megaterium, Bacillus megaterium DSM 509, Synechocystis sp.

J. Biol. Chem.

278

41900-41907

2003

-

2

-

3

-

2

3

-

10

-

2

-

2

-

6

-

2

-

3

-

2

3

-

2

-

2

-

6

-

649817

Schubert

Structural diversity in metal ...

Salmonella enterica subsp. enterica serovar Typhimurium

Biochem. Soc. Trans.

30

595-600

2002

-

1

-

1

-

1

-

4

-

2

-

1

-

1

-

1

-

2

-

649818

Leech

Production of cobalamin and si ...

Bacillus megaterium

Biochem. Soc. Trans.

30

610-613

2002

-

1

-

2

-

1

-

2

-

1

-

1

-

2

-

650801

Raux

Biosynthesis of cobalamin (vit ...

Bacillus megaterium, Clostridioides difficile, Clostridium acetobutylicum, no activity in Bacillus subtilis, no activity in eukaryota, Porphyromonas gingivalis, Pseudomonas aeruginosa, Rhodobacter sphaeroides, Salmonella enterica subsp. enterica serovar Typhimurium

Cell. Mol. Life Sci.

57

1880-1893

2000

-

1

-

7

-

10

1

-

1

-

16

-

1

-

1

-

7

-

1

-

1

-

16

-

644511

Schubert

Common chelatase design in the ...

Salmonella enterica subsp. enterica serovar Typhimurium

Biochemistry

38

10660-10669

1999

-

1

-

1

-

3

-

1

-

3

-

1

-

1

-

1

-

1

-

3

-

651631

Raux

A role for Salmonella typhimur ...

Salmonella enterica subsp. enterica serovar Typhimurium

J. Bacteriol.

179

3202-3212

1997

-

1

-

1

-

4

-

2

-

1

-

1

-

2

-

The AAA(+) motor complex of subunits CobS and CobT of cobaltochelatase visualized by single particle electron microscopy

Data extracted from this reference: