BRENDA - Enzyme Database show
show all sequences of 4.99.1.3

Two distinct roles for two functional cobaltochelatases (CbiK) in Desulfovibrio vulgaris Hildenborough

Lobo, S.A.; Brindley, A.A.; Romao, C.V.; Leech, H.K.; Warren, M.J.; Saraiva, L.M.; Biochemistry 47, 5851-5857 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; expression in Escherichia coli; gene DVU0650, DNA and amino acid sequence determination and analysis, expression of wild-type enzyme and mutant DELTA28DVU0650 in Escherichia coli as His-tagged proteins, complementation of Escherichia coli cysG mutant strain by DELTA28CbiKP; gene DVU1365, DNA and amino acid sequence determination and analysis, expression of wild-type enzyme in Escherichia coli as His-tagged protein, complementation of Escherichia coli cysG mutant strain by CbiKC
Desulfovibrio vulgaris
Engineering
Amino acid exchange
Commentary
Organism
additional information
CbiKc gene is able to complement an Escherichia coli cysG mutant; CbiKp gene is able to complement an Escherichia coli cysG mutant; construction of a truncated DVU0650 protein that starts at amino acid 29, resulting in mutant DELTA28DVU0650. A truncated protein that lacks the signal peptide is also produced, named DELTA28CbiKP
Desulfovibrio vulgaris
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
CbiKc, the isozyme lacks an N-terminal transit peptide; enzyme may be involved in cytoplasmic cobalamin biosynthesis
Desulfovibrio vulgaris
5737
-
periplasm
; CbiKp
Desulfovibrio vulgaris
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
x * 28000, SDS-PAGE and calculated, mature protein
Desulfovibrio vulgaris
31000
-
x * 31000, PAGE and calculated, mature protein; x * 31000, recombinant enzyme, SDS-PAGE
Desulfovibrio vulgaris
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Desulfovibrio vulgaris
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
?
-
-
-
additional information
Desulfovibrio vulgaris
CbiKP is periplasmic located and possibly associated with an iron transport system
?
-
-
-
additional information
Desulfovibrio vulgaris Hildenborough
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
?
-
-
-
additional information
Desulfovibrio vulgaris Hildenborough
CbiKP is periplasmic located and possibly associated with an iron transport system
?
-
-
-
sirohydrochlorin + Co2+
Desulfovibrio vulgaris
a step involved in the vitamin B12 biosynthesis
cobalt-sirohydrochlorin + 2 H+
-
-
?
sirohydrochlorin + Co2+
Desulfovibrio vulgaris Hildenborough
a step involved in the vitamin B12 biosynthesis
cobalt-sirohydrochlorin + 2 H+
-
-
?
sirohydrochlorin + Fe2+
Desulfovibrio vulgaris
-
iron-sirohydrochlorin + 2 H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Desulfovibrio vulgaris
Q72CB8
DVU_1365; isoform CbiKc
-
Desulfovibrio vulgaris Hildenborough
Q72CB8
isoform CbiKc
-
Desulfovibrio vulgaris
Q72EC8
DVU_0650; isoform CbiKp
-
Desulfovibrio vulgaris Hildenborough
Q72EC8
isoform CbiKp
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
enzyme lacks a signal peptide
Desulfovibrio vulgaris
proteolytic modification
processing of 28 amino acid signal peptide
Desulfovibrio vulgaris
Purification (Commentary)
Commentary
Organism
recombinant His-tagged wild-type isozyme and mutant DELTA28DVU0650 from Escherichia coli by nickel affinity chromatography, dialysis, anion exchange chromatography and gel filtration; recombinant His-tagged wild-type isozyme from Escherichia coli by nickel affinity chromatography, dialysis, anion exchange chromatography and gel filtration
Desulfovibrio vulgaris
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0015
-
purified recombinant CbiKc, with Fe2+
Desulfovibrio vulgaris
0.004
-
purified recombinant CbiKc, with Co2+
Desulfovibrio vulgaris
1.5
-
substrate Fe2+, pH 8.0
Desulfovibrio vulgaris
4
-
substrate Co2+, pH 8.0
Desulfovibrio vulgaris
13
-
substrate Fe2+, pH 8.0
Desulfovibrio vulgaris
22
-
substrate Co2+, pH 8.0
Desulfovibrio vulgaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + sirohydrochlorin + Co2+
-
690966
Desulfovibrio vulgaris
ADP + phosphate + Co(II)-sirohydrochlorin + H+
-
-
-
?
ATP + sirohydrochlorin + Co2+
-
690966
Desulfovibrio vulgaris Hildenborough
ADP + phosphate + Co(II)-sirohydrochlorin + H+
-
-
-
?
ATP + sirohydrochlorin + Fe2+
-
690966
Desulfovibrio vulgaris
ADP + phosphate + Fe(II)-sirohydrochlorin + H+
-
-
-
?
ATP + sirohydrochlorin + Fe2+
-
690966
Desulfovibrio vulgaris Hildenborough
ADP + phosphate + Fe(II)-sirohydrochlorin + H+
-
-
-
?
additional information
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
690966
Desulfovibrio vulgaris
?
-
-
-
-
additional information
CbiKP is periplasmic located and possibly associated with an iron transport system
690966
Desulfovibrio vulgaris
?
-
-
-
-
additional information
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
690966
Desulfovibrio vulgaris Hildenborough
?
-
-
-
-
additional information
CbiKP is periplasmic located and possibly associated with an iron transport system
690966
Desulfovibrio vulgaris Hildenborough
?
-
-
-
-
sirohydrochlorin + Co2+
a step involved in the vitamin B12 biosynthesis
690966
Desulfovibrio vulgaris
cobalt-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Co2+
both CbiKP and DELTA28CbiKP insert cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
690966
Desulfovibrio vulgaris
cobalt-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Co2+
the enzyme inserts cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
690966
Desulfovibrio vulgaris
cobalt-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Co2+
a step involved in the vitamin B12 biosynthesis
690966
Desulfovibrio vulgaris Hildenborough
cobalt-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Co2+
the enzyme inserts cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
690966
Desulfovibrio vulgaris Hildenborough
cobalt-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Co2+
both CbiKP and DELTA28CbiKP insert cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
690966
Desulfovibrio vulgaris Hildenborough
cobalt-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Fe2+
-
690966
Desulfovibrio vulgaris
iron-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Fe2+
both CbiKP and DELTA28CbiKP insert cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
690966
Desulfovibrio vulgaris
iron-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Fe2+
the enzyme inserts cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
690966
Desulfovibrio vulgaris
iron-sirohydrochlorin + 2 H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 28000, SDS-PAGE and calculated, mature protein; x * 31000, PAGE and calculated, mature protein; x * 31000, recombinant enzyme, SDS-PAGE
Desulfovibrio vulgaris
Cofactor
Cofactor
Commentary
Organism
Structure
heme
heme b prosthetic group
Desulfovibrio vulgaris
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli; gene DVU0650, DNA and amino acid sequence determination and analysis, expression of wild-type enzyme and mutant DELTA28DVU0650 in Escherichia coli as His-tagged proteins, complementation of Escherichia coli cysG mutant strain by DELTA28CbiKP
Desulfovibrio vulgaris
expression in Escherichia coli; gene DVU1365, DNA and amino acid sequence determination and analysis, expression of wild-type enzyme in Escherichia coli as His-tagged protein, complementation of Escherichia coli cysG mutant strain by CbiKC
Desulfovibrio vulgaris
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
heme b prosthetic group
Desulfovibrio vulgaris
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
CbiKp gene is able to complement an Escherichia coli cysG mutant; construction of a truncated DVU0650 protein that starts at amino acid 29, resulting in mutant DELTA28DVU0650. A truncated protein that lacks the signal peptide is also produced, named DELTA28CbiKP
Desulfovibrio vulgaris
additional information
CbiKc gene is able to complement an Escherichia coli cysG mutant
Desulfovibrio vulgaris
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
CbiKc, the isozyme lacks an N-terminal transit peptide; enzyme may be involved in cytoplasmic cobalamin biosynthesis
Desulfovibrio vulgaris
5737
-
periplasm
; CbiKp
Desulfovibrio vulgaris
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
x * 28000, SDS-PAGE and calculated, mature protein
Desulfovibrio vulgaris
31000
-
x * 31000, PAGE and calculated, mature protein; x * 31000, recombinant enzyme, SDS-PAGE
Desulfovibrio vulgaris
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Desulfovibrio vulgaris
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
?
-
-
-
additional information
Desulfovibrio vulgaris
CbiKP is periplasmic located and possibly associated with an iron transport system
?
-
-
-
additional information
Desulfovibrio vulgaris Hildenborough
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
?
-
-
-
additional information
Desulfovibrio vulgaris Hildenborough
CbiKP is periplasmic located and possibly associated with an iron transport system
?
-
-
-
sirohydrochlorin + Co2+
Desulfovibrio vulgaris
a step involved in the vitamin B12 biosynthesis
cobalt-sirohydrochlorin + 2 H+
-
-
?
sirohydrochlorin + Co2+
Desulfovibrio vulgaris Hildenborough
a step involved in the vitamin B12 biosynthesis
cobalt-sirohydrochlorin + 2 H+
-
-
?
sirohydrochlorin + Fe2+
Desulfovibrio vulgaris
-
iron-sirohydrochlorin + 2 H+
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
enzyme lacks a signal peptide
Desulfovibrio vulgaris
proteolytic modification
processing of 28 amino acid signal peptide
Desulfovibrio vulgaris
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged wild-type isozyme and mutant DELTA28DVU0650 from Escherichia coli by nickel affinity chromatography, dialysis, anion exchange chromatography and gel filtration
Desulfovibrio vulgaris
recombinant His-tagged wild-type isozyme from Escherichia coli by nickel affinity chromatography, dialysis, anion exchange chromatography and gel filtration
Desulfovibrio vulgaris
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0015
-
purified recombinant CbiKc, with Fe2+
Desulfovibrio vulgaris
0.004
-
purified recombinant CbiKc, with Co2+
Desulfovibrio vulgaris
1.5
-
substrate Fe2+, pH 8.0
Desulfovibrio vulgaris
4
-
substrate Co2+, pH 8.0
Desulfovibrio vulgaris
13
-
substrate Fe2+, pH 8.0
Desulfovibrio vulgaris
22
-
substrate Co2+, pH 8.0
Desulfovibrio vulgaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + sirohydrochlorin + Co2+
-
690966
Desulfovibrio vulgaris
ADP + phosphate + Co(II)-sirohydrochlorin + H+
-
-
-
?
ATP + sirohydrochlorin + Co2+
-
690966
Desulfovibrio vulgaris Hildenborough
ADP + phosphate + Co(II)-sirohydrochlorin + H+
-
-
-
?
ATP + sirohydrochlorin + Fe2+
-
690966
Desulfovibrio vulgaris
ADP + phosphate + Fe(II)-sirohydrochlorin + H+
-
-
-
?
ATP + sirohydrochlorin + Fe2+
-
690966
Desulfovibrio vulgaris Hildenborough
ADP + phosphate + Fe(II)-sirohydrochlorin + H+
-
-
-
?
additional information
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
690966
Desulfovibrio vulgaris
?
-
-
-
-
additional information
CbiKP is periplasmic located and possibly associated with an iron transport system
690966
Desulfovibrio vulgaris
?
-
-
-
-
additional information
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
690966
Desulfovibrio vulgaris Hildenborough
?
-
-
-
-
additional information
CbiKP is periplasmic located and possibly associated with an iron transport system
690966
Desulfovibrio vulgaris Hildenborough
?
-
-
-
-
sirohydrochlorin + Co2+
a step involved in the vitamin B12 biosynthesis
690966
Desulfovibrio vulgaris
cobalt-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Co2+
both CbiKP and DELTA28CbiKP insert cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
690966
Desulfovibrio vulgaris
cobalt-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Co2+
the enzyme inserts cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
690966
Desulfovibrio vulgaris
cobalt-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Co2+
a step involved in the vitamin B12 biosynthesis
690966
Desulfovibrio vulgaris Hildenborough
cobalt-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Co2+
the enzyme inserts cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
690966
Desulfovibrio vulgaris Hildenborough
cobalt-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Co2+
both CbiKP and DELTA28CbiKP insert cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
690966
Desulfovibrio vulgaris Hildenborough
cobalt-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Fe2+
-
690966
Desulfovibrio vulgaris
iron-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Fe2+
both CbiKP and DELTA28CbiKP insert cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
690966
Desulfovibrio vulgaris
iron-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Fe2+
the enzyme inserts cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
690966
Desulfovibrio vulgaris
iron-sirohydrochlorin + 2 H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 28000, SDS-PAGE and calculated, mature protein
Desulfovibrio vulgaris
?
x * 31000, PAGE and calculated, mature protein; x * 31000, recombinant enzyme, SDS-PAGE
Desulfovibrio vulgaris
Other publictions for EC 4.99.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747658
Lobo
Desulfovibrio vulgaris CbiKP ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris DSM 644
Environ. Microbiol.
19
106-118
2017
-
-
1
1
7
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
7
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730419
Bali
Identification and characteriz ...
Paracoccus denitrificans, Paracoccus pantotrophus
Mol. Microbiol.
92
153-163
2014
-
-
1
-
2
-
-
-
-
-
1
2
-
4
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730479
Moore
Towards a cell factory for vit ...
Bacillus megaterium, Bacillus megaterium DSM 319
New Biotechnol.
31
553-561
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
716791
Romao
Evolution in a family of chela ...
Archaeoglobus fulgidus, Desulfovibrio vulgaris, Salmonella enterica
Proc. Natl. Acad. Sci. USA
108
97-102
2011
-
-
1
1
-
-
-
-
2
-
-
-
-
3
-
-
1
-
-
-
-
-
3
3
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
1
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699853
Lundqvist
The AAA(+) motor complex of su ...
Brucella melitensis
J. Struct. Biol.
167
227-234
2009
-
-
1
-
-
-
-
-
-
-
3
1
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690966
Lobo
Two distinct roles for two fun ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris Hildenborough
Biochemistry
47
5851-5857
2008
-
-
1
-
1
-
-
-
2
-
2
10
-
4
-
2
1
-
-
-
6
-
24
1
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
2
-
-
-
-
-
2
-
2
10
-
-
2
2
-
-
6
-
24
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682635
Pisarchik
Probing the structural plastic ...
Methanosarcina barkeri
Protein Eng. Des. Sel.
20
257-265
2007
-
-
1
1
3
-
-
2
-
-
11
-
-
2
-
-
1
-
-
-
11
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
3
-
-
-
-
2
-
-
11
-
-
-
-
1
-
-
11
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681658
Yin
Crystal structure of the vitam ...
Archaeoglobus fulgidus
J. Struct. Funct. Genomics
7
37-50
2006
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
2
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12
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Characterization of the cobalt ...
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6
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Structural diversity in metal ...
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Leech
Production of cobalamin and si ...
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650801
Raux
Biosynthesis of cobalamin (vit ...
Bacillus megaterium, Clostridioides difficile, Clostridium acetobutylicum, no activity in Bacillus subtilis, no activity in eukaryota, Porphyromonas gingivalis, Pseudomonas aeruginosa, Rhodobacter sphaeroides, Salmonella enterica subsp. enterica serovar Typhimurium
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644511
Schubert
Common chelatase design in the ...
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Raux
A role for Salmonella typhimur ...
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1997
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