Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli as non His-tagged protein, as His-tagged protein fusion protein and as selenomethionine-substituted protein | Archaeoglobus fulgidus |
Crystallization (Comment) | Organism |
---|---|
using the sitting drop vapor diffusion technique in 96-well plates, in two different crystal forms consisting of a central mixed b-sheet flanked by four alpha helices | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O29537 | - |
- |
Purification (Comment) | Organism |
---|---|
by using a metal chelating affinity column charged with Ni2+ or a chelating Sepharose-column charged with Co2+ | Archaeoglobus fulgidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004 | - |
non His-tagged protein | Archaeoglobus fulgidus |
0.017 | - |
His-tagged protein | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Co2+ | - |
Archaeoglobus fulgidus | cobalt-sirohydrochlorin + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CbiXS | - |
Archaeoglobus fulgidus |
sirohydrochlorin cobalt-lyase | - |
Archaeoglobus fulgidus |
sirohydrochlorin cobaltochelatase | - |
Archaeoglobus fulgidus |