Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.99.1.3 extracted from

  • Yin, J.; Xu, L.X.; Cherney, M.M.; Raux-Deery, E.; Bindley, A.A.; Savchenko, A.; Walker, J.R.; Cuff, M.E.; Warren, M.J.; James, M.N.
    Crystal structure of the vitamin B12 biosynthetic cobaltochelatase, CbiXS, from Archaeoglobus fulgidus (2006), J. Struct. Funct. Genomics, 7, 37-50.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli as non His-tagged protein, as His-tagged protein fusion protein and as selenomethionine-substituted protein Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment) Organism
using the sitting drop vapor diffusion technique in 96-well plates, in two different crystal forms consisting of a central mixed b-sheet flanked by four alpha helices Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O29537
-
-

Purification (Commentary)

Purification (Comment) Organism
by using a metal chelating affinity column charged with Ni2+ or a chelating Sepharose-column charged with Co2+ Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.004
-
non His-tagged protein Archaeoglobus fulgidus
0.017
-
His-tagged protein Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sirohydrochlorin + Co2+
-
Archaeoglobus fulgidus cobalt-sirohydrochlorin + 2 H+
-
?

Synonyms

Synonyms Comment Organism
CbiXS
-
Archaeoglobus fulgidus
sirohydrochlorin cobalt-lyase
-
Archaeoglobus fulgidus
sirohydrochlorin cobaltochelatase
-
Archaeoglobus fulgidus