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Literature summary for 4.99.1.3 extracted from

  • Raux, E.; Schubert, H.L.; Warren, M.J.
    Biosynthesis of cobalamin (vitamin B12): a bacterial conundrum (2000), Cell. Mol. Life Sci., 57, 1880-1893.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned and overexpressed Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sirohydrochlorin + Co2+ Priestia megaterium cobalamin biosynthesis cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ Clostridium acetobutylicum cobalamin biosynthesis cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ Porphyromonas gingivalis cobalamin biosynthesis cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ Clostridioides difficile cobalamin biosynthesis cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ Cereibacter sphaeroides cobalamin biosynthesis, oxygen-independent pathway cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ Pseudomonas aeruginosa cobalamin biosynthesis, oxygen-independent pathway cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ Salmonella enterica subsp. enterica serovar Typhimurium cobalamin biosynthesis, oxygen-independent route, CbiK is a biglobal enzyme containing 2 a/b domains, which generate an active site with a deep rectangular cleft at their interface cobaltsirohydrochlorin + H+
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-
Clostridioides difficile
-
-
-
Clostridium acetobutylicum
-
-
-
no activity in Bacillus subtilis
-
-
-
no activity in eukaryota
-
-
-
Porphyromonas gingivalis
-
-
-
Priestia megaterium
-
-
-
Pseudomonas aeruginosa
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Oxidation Stability

Oxidation Stability Organism
requires molecular oxygen Pseudomonas aeruginosa

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CbiK fused together with CbiL as a multifunctional protein Clostridioides difficile ?
-
?
precorrin-2 + Co2+
-
Pseudomonas aeruginosa Co-precorrin-2 + H+
-
?
sirohydrochlorin + Co2+
-
Salmonella enterica subsp. enterica serovar Typhimurium cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+
-
Cereibacter sphaeroides cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+
-
Priestia megaterium cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+
-
Pseudomonas aeruginosa cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+
-
Clostridium acetobutylicum cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+
-
Porphyromonas gingivalis cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+
-
Clostridioides difficile cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ cobalamin biosynthesis Priestia megaterium cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ cobalamin biosynthesis Clostridium acetobutylicum cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ cobalamin biosynthesis Porphyromonas gingivalis cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ cobalamin biosynthesis Clostridioides difficile cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ cobalamin biosynthesis, oxygen-independent pathway Cereibacter sphaeroides cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ cobalamin biosynthesis, oxygen-independent pathway Pseudomonas aeruginosa cobaltsirohydrochlorin + H+
-
?
sirohydrochlorin + Co2+ cobalamin biosynthesis, oxygen-independent route, CbiK is a biglobal enzyme containing 2 a/b domains, which generate an active site with a deep rectangular cleft at their interface Salmonella enterica subsp. enterica serovar Typhimurium cobaltsirohydrochlorin + H+
-
?

Synonyms

Synonyms Comment Organism
CbiK
-
Salmonella enterica subsp. enterica serovar Typhimurium
CbiK
-
Cereibacter sphaeroides
CbiK
-
Priestia megaterium
CbiK
-
Pseudomonas aeruginosa
CbiK
-
Clostridium acetobutylicum
CbiK
-
Porphyromonas gingivalis
CbiK
-
Clostridioides difficile
cobalt chelatase
-
Salmonella enterica subsp. enterica serovar Typhimurium
cobalt chelatase
-
Cereibacter sphaeroides
cobalt chelatase
-
Priestia megaterium
cobalt chelatase
-
Pseudomonas aeruginosa
cobalt chelatase
-
Clostridium acetobutylicum
cobalt chelatase
-
Porphyromonas gingivalis
cobalt chelatase
-
Clostridioides difficile