BRENDA - Enzyme Database show
show all sequences of 4.99.1.3

Common chelatase design in the branched tetrapyrrole pathways of heme and anaerobic cobalamin synthesis

Schubert, H.L.; Raux, E.; Wilson, K.S.; Warren, M.J.; Biochemistry 38, 10660-10669 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpressed in Escherichia coli BL21(DE3)pLys cells by cloning the gene into pET14b
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary)
Crystallization
Organism
hexagonal crystals are grown using the hanging-drop method, crystals are in space group P6(3)22 with cell dimensions a = b = 128.08 A, c = 85.44 A
Salmonella enterica subsp. enterica serovar Typhimurium
Engineering
Amino acid exchange
Commentary
Organism
H145A/H207A
site-directed mutagenesis
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sirohydrochlorin + Co2+
Salmonella enterica subsp. enterica serovar Typhimurium
cobalamin branched biosynthetic pathway
cobalt-sirohydrochlorin + H+
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Purification (Commentary)
Commentary
Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
precorrin-2 + Co2+
-
644511
Salmonella enterica subsp. enterica serovar Typhimurium
cobalt-precorrin-2 + H+
-
644511
Salmonella enterica subsp. enterica serovar Typhimurium
?
sirohydrochlorin + Co2+
-
644511
Salmonella enterica subsp. enterica serovar Typhimurium
cobalt-sirohydrochlorin + H+
-
644511
Salmonella enterica subsp. enterica serovar Typhimurium
?
sirohydrochlorin + Co2+
cobalamin branched biosynthetic pathway
644511
Salmonella enterica subsp. enterica serovar Typhimurium
cobalt-sirohydrochlorin + H+
-
644511
Salmonella enterica subsp. enterica serovar Typhimurium
?
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpressed in Escherichia coli BL21(DE3)pLys cells by cloning the gene into pET14b
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hexagonal crystals are grown using the hanging-drop method, crystals are in space group P6(3)22 with cell dimensions a = b = 128.08 A, c = 85.44 A
Salmonella enterica subsp. enterica serovar Typhimurium
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H145A/H207A
site-directed mutagenesis
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sirohydrochlorin + Co2+
Salmonella enterica subsp. enterica serovar Typhimurium
cobalamin branched biosynthetic pathway
cobalt-sirohydrochlorin + H+
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
precorrin-2 + Co2+
-
644511
Salmonella enterica subsp. enterica serovar Typhimurium
cobalt-precorrin-2 + H+
-
644511
Salmonella enterica subsp. enterica serovar Typhimurium
?
sirohydrochlorin + Co2+
-
644511
Salmonella enterica subsp. enterica serovar Typhimurium
cobalt-sirohydrochlorin + H+
-
644511
Salmonella enterica subsp. enterica serovar Typhimurium
?
sirohydrochlorin + Co2+
cobalamin branched biosynthetic pathway
644511
Salmonella enterica subsp. enterica serovar Typhimurium
cobalt-sirohydrochlorin + H+
-
644511
Salmonella enterica subsp. enterica serovar Typhimurium
?
Other publictions for EC 4.99.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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2
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1
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1
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716791
Romao
Evolution in a family of chela ...
Archaeoglobus fulgidus, Desulfovibrio vulgaris, Salmonella enterica
Proc. Natl. Acad. Sci. USA
108
97-102
2011
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-
1
1
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2
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3
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1
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3
3
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3
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1
3
1
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1
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3
3
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699853
Lundqvist
The AAA(+) motor complex of su ...
Brucella melitensis
J. Struct. Biol.
167
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2009
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1
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3
1
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1
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2
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1
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1
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1
2
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690966
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Biochemistry
47
5851-5857
2008
-
-
1
-
1
-
-
-
2
-
2
10
-
4
-
2
1
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6
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24
1
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1
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2
1
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2
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2
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2
10
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2
2
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6
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24
2
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682635
Pisarchik
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Methanosarcina barkeri
Protein Eng. Des. Sel.
20
257-265
2007
-
-
1
1
3
-
-
2
-
-
11
-
-
2
-
-
1
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11
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1
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1
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1
3
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2
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11
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1
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11
-
1
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681658
Yin
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Archaeoglobus fulgidus
J. Struct. Funct. Genomics
7
37-50
2006
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1
1
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1
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1
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2
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1
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1
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1
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644512
Brindley
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J. Biol. Chem.
278
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2003
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2
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5
6
6
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17
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2
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3
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12
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2
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5
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6
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3
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12
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652468
Leech
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Bacillus megaterium, Bacillus megaterium DSM 509, Synechocystis sp.
J. Biol. Chem.
278
41900-41907
2003
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2
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3
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2
2
3
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10
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2
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6
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2
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6
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649817
Schubert
Structural diversity in metal ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochem. Soc. Trans.
30
595-600
2002
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1
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1
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1
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4
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2
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649818
Leech
Production of cobalamin and si ...
Bacillus megaterium
Biochem. Soc. Trans.
30
610-613
2002
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1
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2
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650801
Raux
Biosynthesis of cobalamin (vit ...
Bacillus megaterium, Clostridioides difficile, Clostridium acetobutylicum, no activity in Bacillus subtilis, no activity in eukaryota, Porphyromonas gingivalis, Pseudomonas aeruginosa, Rhodobacter sphaeroides, Salmonella enterica subsp. enterica serovar Typhimurium
Cell. Mol. Life Sci.
57
1880-1893
2000
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1
1
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7
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10
1
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1
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16
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1
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1
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7
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1
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1
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16
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644511
Schubert
Common chelatase design in the ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochemistry
38
10660-10669
1999
-
-
1
1
1
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-
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1
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3
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1
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3
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651631
Raux
A role for Salmonella typhimur ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
179
3202-3212
1997
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