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Literature summary for 4.99.1.3 extracted from

  • Schubert, H.L.; Raux, E.; Wilson, K.S.; Warren, M.J.
    Common chelatase design in the branched tetrapyrrole pathways of heme and anaerobic cobalamin synthesis (1999), Biochemistry, 38, 10660-10669.
    View publication on PubMed
Search for more literature for 4.99.1.3

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli BL21(DE3)pLys cells by cloning the gene into pET14b Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
hexagonal crystals are grown using the hanging-drop method, crystals are in space group P6(3)22 with cell dimensions a = b = 128.08 A, c = 85.44 A Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
H145A/H207A site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sirohydrochlorin + Co2+ Salmonella enterica subsp. enterica serovar Typhimurium cobalamin branched biosynthetic pathway cobalt-sirohydrochlorin + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
precorrin-2 + Co2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium cobalt-precorrin-2 + H+
-
 ?
sirohydrochlorin + Co2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium cobalt-sirohydrochlorin + H+
-
 ?
sirohydrochlorin + Co2+ cobalamin branched biosynthetic pathway Salmonella enterica subsp. enterica serovar Typhimurium cobalt-sirohydrochlorin + H+
-
 ?

Synonyms

Synonyms Comment Organism
anaerobic cobalt chelatase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
CbiK
-
 Salmonella enterica subsp. enterica serovar Typhimurium