Application | Comment | Organism |
---|---|---|
environmental protection | a new transgenic tobacco plant for phytoremediation of CH3Hg+ pollution: The new ppk/merT/merB-transgenic tobacco plant, which contains the integrated bacterial merB gene encoding MerB, has the ability to tolerate and accumulate high levels of Hg2+ inside the plant cells from simulated soils, probably via a chelation mechanism of polyP with Hg2+, without releasing mercury vapor into the atmosphere | Pseudomonas sp. K-62 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | The organomercurial lyase gene of plasmid pMR26 (GenBank/DDBJ accession no. D83080) is amplified by PCR using two specific primers. The pBMK01 plasmid is transformed into Agrobacterium tumefaciens LBA4404 by electroporation, and its sequence is confirmed. The ppk/merT-transgenic tobacco (T3) is then transformed with this Agrobacterium tumefaciens, the tobacco callus shows more resistance to CH3Hg+ and accumulated significantly more mercury from CH3Hg+-containing medium than does the ppk/merT-transgenic and wild-type tobaccos | Pseudomonas sp. K-62 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CH3Hg+ | the growth rates of both the wild-type and ppk/merT-transgenic tobacco callus are largely inhibited by CH3Hg+ in a dose-dependent manner | Pseudomonas sp. K-62 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. K-62 | O07303 | The organomercurial lyase gene of plasmid pMR26 (GenBank/DDBJ accession no. D83080) is amplified by PCR using two specific primers. The pBMK01 plasmid is transformed into Agrobacterium tumefaciens LBA4404 by electroporation, and its sequence is confirmed. The ppk/merT-transgenic tobacco (T3) is then transformed with this Agrobacterium tumefaciens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CH3Hg+ + H+ | the growth rates of both the wild-type and ppk/merTtransgenic tobacco callus are largely inhibited by CH3Hg+ in a dose-dependent manner | Pseudomonas sp. K-62 | Hg2+ + CH4 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
merB | - |
Pseudomonas sp. K-62 |
organomercurial lyase | - |
Pseudomonas sp. K-62 |