Protein Variants | Comment | Organism |
---|---|---|
F323L | the mutant exhibits an enhanced Ni2+-chelatase activity over that of the parent wild type ferrochelatase | Rattus norvegicus |
S143T | the mutant exhibits an enhanced Ni2+-chelatase activity over that of the parent wild type ferrochelatase | Rattus norvegicus |
S143T/F323L | the mutant exhibits an enhanced Ni2+-chelatase activity over that of the parent wild type ferrochelatase | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no Ni2+ substrate inhibition of ferrochelatase is observed | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00026 | - |
protoporphyrin IX | wild type enzyme, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.00029 | - |
protoporphyrin IX | mutant enzyme S143T, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.00123 | - |
protoporphyrin IX | mutant enzyme F323L, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.00172 | - |
protoporphyrin IX | wild type enzyme, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.00226 | - |
Fe2+ | wild type enzyme, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.00243 | - |
protoporphyrin IX | mutant enzyme S143T/F323L, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.00291 | - |
Fe2+ | mutant enzyme S143T, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.00547 | - |
protoporphyrin IX | mutant enzyme F323L, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.00558 | - |
protoporphyrin IX | mutant enzyme S143T/F323L, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.00728 | - |
Fe2+ | mutant enzyme S143T/F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.00831 | - |
protoporphyrin IX | mutant enzyme S143T, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.00895 | - |
Fe2+ | mutant enzyme F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.02206 | - |
Ni2+ | wild type enzyme, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.03218 | - |
Ni2+ | mutant enzyme F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.06521 | - |
Ni2+ | mutant enzyme S143T, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.1037 | - |
Ni2+ | mutant enzyme S143T/F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrin IX + Fe2+ | Rattus norvegicus | - |
protoheme IX + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrin IX + Fe2+ | - |
Rattus norvegicus | protoheme IX + 2 H+ | - |
? | |
protoporphyrin IX + Ni2+ | - |
Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
protoheme lyase | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0125 | - |
protoporphyrin IX | wild type enzyme, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.142 | - |
protoporphyrin IX | wild type enzyme, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.212 | - |
protoporphyrin IX | mutant enzyme F323L, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.44 | - |
protoporphyrin IX | mutant enzyme S143T/F323L, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
0.59 | - |
protoporphyrin IX | mutant enzyme S143T, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
1.21 | - |
protoporphyrin IX | mutant enzyme S143T, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
1.6 | - |
protoporphyrin IX | mutant enzyme F323L, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
1.9 | - |
protoporphyrin IX | mutant enzyme S143T/F323L, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
Ni2+ | wild type enzyme, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
1.605 | - |
Ni2+ | mutant enzyme F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
4.23 | - |
Ni2+ | mutant enzyme S143T/F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
9.05 | - |
Ni2+ | mutant enzyme S143T, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
47.3 | - |
protoporphyrin IX | wild type enzyme, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
62.5 | - |
Fe2+ | wild type enzyme, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
82.3 | - |
protoporphyrin IX | wild type enzyme, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
145.7 | - |
protoporphyrin IX | mutant enzyme S143T, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
173.3 | - |
protoporphyrin IX | mutant enzyme F323L, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
179 | - |
Fe2+ | mutant enzyme F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
180 | - |
protoporphyrin IX | mutant enzyme S143T/F323L, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
261.3 | - |
Fe2+ | mutant enzyme S143T/F323L, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
292.8 | - |
protoporphyrin IX | mutant enzyme F323L, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
340.5 | - |
protoporphyrin IX | mutant enzyme S143T/F323L, with Fe2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
416.8 | - |
Fe2+ | mutant enzyme S143T, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus | |
2015 | - |
protoporphyrin IX | mutant enzyme S143T, with Ni2+ as cosubstrate, 0.1 M Tris-acetate, pH 8.0, 0.5 M NaCl, 0.1% (v/v) Tween 80, at 30°C | Rattus norvegicus |