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Literature summary for 4.98.1.1 extracted from

  • Kwon, S.J.; Petri, R.; DeBoer, A.L.; Schmidt-Dannert, C.
    A high-throughput screen for porphyrin metal chelatases: application to the directed evolution of ferrochelatases for metalloporphyrin biosynthesis (2004), ChemBioChem, 5, 1069-1074.
    View publication on PubMed
Search for more literature for 4.98.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
D76G/K102T lower KM-value than the wild-type with protoporphyrin IX as substrate, higher KM-value than the wild-type with Fe2+ as substrate Bacillus subtilis
E61K lower KM-value than the wild-type with protoporphyrin IX as substrate, higher KM-value than the wild-type with Fe2+ as substrate Bacillus subtilis
E61K/L185Q/G212D lower KM-value than the wild-type with protoporphyrin IX as substrate, higher KM-value than the wild-type with Fe2+ as substrate Bacillus subtilis
M11V/G104A higher KM-value than the wild-type with protoporphyrin IX as substrate, lower KM-value than the wild-type with Fe2+ as substrate Bacillus subtilis
R31G lower KM-values than the wild-type Bacillus subtilis
T302A lower KM-value than the wild-type with protoporphyrin IX as substrate, higher KM-value than the wild-type with Fe2+ as substrate Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00044
-
 protoporphyrin IX mutant D76G/K102T, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.00078
-
 protoporphyrin IX mutant E61K, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.00079
-
 protoporphyrin IX mutant T302A, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.00083
-
 protoporphyrin IX R31G, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.00086
-
 protoporphyrin IX E61K/L185Q/G212D, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.00091
-
 protoporphyrin IX wild-type, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.0013
-
 protoporphyrin IX M11V/G104A, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.0094
-
 Fe2+ M11V/G104A, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.01
-
 Fe2+ R31G, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.013
-
 Fe2+ wild-type, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.014
-
 Fe2+ E61K/L185Q/G212D, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.017
-
 Fe2+ mutant E61K, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.019
-
 Fe2+ mutant T302A, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.021
-
 Fe2+ mutant D76G/K102T, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrin + Fe2+ Bacillus subtilis
-
 protoheme + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P32396
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.033
-
 R31G, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.041
-
 wild-type, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.054
-
 mutant T302A, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.056
-
 mutant D76G/K102T, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.065
-
 M11V/G104A, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.067
-
 mutant E61K, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.093
-
 E61K/L185Q/G212D, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoporphyrin + Fe2+
-
 Bacillus subtilis protoheme + H+
-
 ?
protoporphyrin IX + Fe2+ pH 7.6, 30°C, unaerobic conditions Bacillus subtilis protoheme + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
ferrochelatase
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 Fe2+ R31G, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.023
-
 Fe2+ wild-type, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.032
-
 Fe2+ mutant T302A, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.033
-
 Fe2+ mutant D76G/K102T, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.038
-
 Fe2+ M11V/G104A, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.04
-
 Fe2+ mutant E61K, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis
0.055
-
 Fe2+ E61K/L185Q/G212D, pH 7.6, 30°C, unaerobic conditions Bacillus subtilis