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Literature summary for 4.6.1.22 extracted from

  • Bugrysheva, J.; Scott, J.
    The ribonucleases J1 and J2 are essential for growth and have independent roles in mRNA decay in Streptococcus pyogenes (2010), Mol. Microbiol., 75, 731-743.
    View publication on PubMed
Search for more literature for 4.6.1.22

Cloned(Commentary)

Cloned (Comment) Organism
gene rnjA, DNA and amino acid sequence determination and analysis, sequence comparison to Bacillus subtilis enzyme, phylogenetic analysis Streptococcus pyogenes
gene rnjB, DNA and amino acid sequence determination and analysis, sequence comparison to Bacillus subtilis enzyme, phylogenetic analysis Streptococcus pyogenes

Protein Variants

Protein Variants Comment Organism
additional information construction of a tetracycline-inducible mutant for RNase J1 from strain MGAS315, conditional mutant JRS7316, the enzyme-deficient strain shows growth defects, phenotype overview Streptococcus pyogenes
additional information construction of a tetracycline-inducible mutant for RNase J2 from strain MGAS315, the enzyme-deficient strain shows growth defects, phenotype overview Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptococcus pyogenes ribonucleases J1 and J2 have both endoribonucleolytic and 5'-to-3' exoribonucleolytic activities. Both act on class I and class II mRNAs, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes Q8K5W8 RNase J1; serotype M3, i.e. group A streptococcus or GAS, gene rnjA encodes RNase J1
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Streptococcus pyogenes Q8K7S6 RNase J2; serotype M3, i.e. group A streptococcus or GAS, gene rnjB encodes RNase J2
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Source Tissue

Source Tissue Comment Organism Textmining
additional information decay of class I and class II messages are affected differently by RNases J1 and J2 in exponential phase, both RNases increase the decay rate for class I messages which are degraded rapidly in exponential phase, but show a delay phase before decay of class II messages is initiated, overview Streptococcus pyogenes
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ribonucleases J1 and J2 have both endoribonucleolytic and 5'-to-3' exoribonucleolytic activities. Both act on class I and class II mRNAs, overview Streptococcus pyogenes ?
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 ?
additional information active on hasA and gyrA class I mRNAs and sagA and sda class II mRNAs Streptococcus pyogenes ?
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 ?

Synonyms

Synonyms Comment Organism
ribonuclease J1
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 Streptococcus pyogenes
ribonuclease J2
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 Streptococcus pyogenes
RNase J1
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 Streptococcus pyogenes
RNase J2
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 Streptococcus pyogenes

General Information

General Information Comment Organism
evolution the enzyme belongs to the beta-CASP subfamily of metallo-beta-lactamases Streptococcus pyogenes
malfunction conditional mutants for both RNases J1 and J2 require induction for growth Streptococcus pyogenes
physiological function ribonucleases J1 and J2 participate in degradation and regulatory processing of mRNA. mRNA decay is important in regulation of virulence factors of Streptococcus pyogenes, both of these RNases are essential for growth of the organism. RNases J1 and J2 affect the rate of decay of class I messages and the length of the first phase in decay of class II messages, overview Streptococcus pyogenes