Literature summary for 4.6.1.22 extracted from

Jamalli, A.; Hebert, A.; Zig, L.; Putzer, H.
Control of expression of the RNases J1 and J2 in Bacillus subtilis (2014), J. Bacteriol., 196, 318-324.

Cloned(Commentary)

Cloned (Comment)	Organism
gene rnjB is transcribed constitutively from a sigma A promoter. Gene rnjA is transcribed as a bicistronic transcript from a single promoter, optimal expression of RNase J1 from gene rnjA requires cotranscription and cotranslation with the upstream ykzG gene, sequence and regulatory elements upstream of the rnjA open reading frame, overview. In the absence of coupled translation, RNase J1 expression is decreased more than 5fold, transcription of the ykzG operon initiates at a sigma A promoter with a noncanonical35 box that is required for optimal transcription. In Bacillus subtilis strain SSB356, the rnjA gene is under the control of the xylose-inducible Pxyl promoter	Bacillus subtilis

Cloned (Comment)

Organism

gene rnjB is transcribed constitutively from a sigma A promoter. Gene rnjA is transcribed as a bicistronic transcript from a single promoter, optimal expression of RNase J1 from gene rnjA requires cotranscription and cotranslation with the upstream ykzG gene, sequence and regulatory elements upstream of the rnjA open reading frame, overview. In the absence of coupled translation, RNase J1 expression is decreased more than 5fold, transcription of the ykzG operon initiates at a sigma A promoter with a noncanonical35 box that is required for optimal transcription. In Bacillus subtilis strain SSB356, the rnjA gene is under the control of the xylose-inducible Pxyl promoter

Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
additional information	depletion of RNase J1 is achieved by growing the cells without inducer until a decrease in growth rate becomes perceptible	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	gene rnjA encoding RNase J1, anf gene rnjB encoding RNase J2	-
Bacillus subtilis SSB1002	-	gene rnjA encoding RNase J1, anf gene rnjB encoding RNase J2	-

Synonyms

Synonyms	Comment	Organism
endoribonuclease J1	-	Bacillus subtilis
endoribonuclease J2	-	Bacillus subtilis
RNase J1	-	Bacillus subtilis
RNase J2	-	Bacillus subtilis

Expression

Organism	Comment	Expression
Bacillus subtilis	biosynthesis of enzyme RNase J1 is autocontrolled within a small range (1.4fold) and also slightly stimulated (1.4fold) in the absence of enzyme RNase J2	up

General Information

General Information	Comment	Organism
physiological function	in Bacillus subtilis, the dual activity 5' exo- and endoribonucleases J1 and J2 are important players in mRNA and stable RNA maturation and degradation. The two nucleases in the cell primarily act as a heterodimer in vivo	Bacillus subtilis