Literature summary for 4.6.1.22 extracted from

Yao, S.; Sharp, J.S.; Bechhofer, D.H.
Bacillus subtilis RNase J1 endonuclease and 5 exonuclease activities in the turnover of DeltaermC mRNA (2009), RNA, 15, 2331-2339.

Cloned(Commentary)

Cloned (Comment)	Organism
chromosomal DNA from the RNase J1 conditional mutant strain used to transform Bacillus subtilis host BG1 to erythromycin resistance. The RNase J1 conditional strain also contains plasmid pMAP65, which carries extra copies of the lacI gene. Preparation and transformation of Bacillus subtilis competent cell cultures. RNase J1 transcription under control of an IPTG-inducible promoter in the RNase J1 conditional mutant strain	Bacillus subtilis

Cloned (Comment)

Organism

chromosomal DNA from the RNase J1 conditional mutant strain used to transform Bacillus subtilis host BG1 to erythromycin resistance. The RNase J1 conditional strain also contains plasmid pMAP65, which carries extra copies of the lacI gene. Preparation and transformation of Bacillus subtilis competent cell cultures. RNase J1 transcription under control of an IPTG-inducible promoter in the RNase J1 conditional mutant strain

Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DELTAermC mRNA + H2O	DELTAermC mRNA decay is RNase J1 dependent. Decay-initiating endonuclease cleavage can occur at several sites near the 3' end. Preferred RNase J1 target sites are located in the downstream half of DELTAermC mRNA, located upstream of eSL1 (ermC stem-loop 1), between eSL1 and eSL2, and between eSL2 and the 3' transcription terminator. The putative endonuclease cleavages in the body of the message are not dependent on ribosome flow. Even in the absence of these sites, stability is further increased in a strain with reduced RNase J1, suggesting alternate pathways for decay that can include exonucleolytic decay from the 5' end	Bacillus subtilis	?	-	?

Synonyms

Synonyms	Comment	Organism
RNase J1	-	Bacillus subtilis

General Information

General Information	Comment	Organism
malfunction	DELTAermC mRNA shows increased stability in a RNase J1 mutant strain, that contains reduced level of RNase J1. Insertion of a strong stem-loop structure at +65 results in increased stability. Weakening this stem-loop structure results in reversion to wild-type stability. RNA fragments containing the 3' end are detected in a strain with reduced RNase J1 expression, but are undetectable in the wild-type. The 5' ends of these fragments map to the upstream side of predicted stem-loop structures, consistent with an impediment to RNase J1 5' exonuclease processivity. RNase J1 is involved even in decay of the RNA encoded by a 129-nt deletion construct, perhaps by a 59-to-39 exonucleolytic pathway or by cleavage at sites in the upstream half of the RNA. Deletion of the three target sites results in increased mRNA half-life (ca. 15 min)	Bacillus subtilis