Application | Comment | Organism |
---|---|---|
analysis | - |
Enterobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24300 | 25500 | gel filtration, MW depending on purification procedure | Enterobacter sp. |
28500 | 31500 | sucrose density gradient centrifugation, MW depending on purification procedure | Enterobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA + H2O | Enterobacter sp. | preferential cleavage at CpA | nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Enterobacter sp. |
Storage Stability | Organism |
---|---|
-20°C, 0.1 mg protein per ml Tris-HCL elution buffer, stable for about 3 weeks | Enterobacter sp. |
-20°C, lyophilized enzyme, stable for about 3 weeks | Enterobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA + H2O | preferential cleavage at CpA | Enterobacter sp. | nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
for 15 min, 100% activity | Enterobacter sp. |
65 | - |
for 15 min, 78-80% activity depending on purification procedure | Enterobacter sp. |
75 | - |
for 15 min, 53-57% activity depending on purification procedure | Enterobacter sp. |
85 | - |
for 15 min, 18-20% activity depending on purification procedure | Enterobacter sp. |
100 | - |
for 15 min, total loss of activity | Enterobacter sp. |