Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.21 extracted from

  • Levy, C.C.
    Enterobacter ribonuclease. II. A reexamination of the enzymes specificity toward internucleotide linkages (1971), Biochim. Biophys. Acta, 246, 476-486.
    View publication on PubMed

Application

Application Comment Organism
analysis
-
Enterobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24300 25500 gel filtration, MW depending on purification procedure Enterobacter sp.
28500 31500 sucrose density gradient centrifugation, MW depending on purification procedure Enterobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA + H2O Enterobacter sp. preferential cleavage at CpA nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates
-
?

Organism

Organism UniProt Comment Textmining
Enterobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Enterobacter sp.

Storage Stability

Storage Stability Organism
-20°C, 0.1 mg protein per ml Tris-HCL elution buffer, stable for about 3 weeks Enterobacter sp.
-20°C, lyophilized enzyme, stable for about 3 weeks Enterobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA + H2O preferential cleavage at CpA Enterobacter sp. nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
for 15 min, 100% activity Enterobacter sp.
65
-
for 15 min, 78-80% activity depending on purification procedure Enterobacter sp.
75
-
for 15 min, 53-57% activity depending on purification procedure Enterobacter sp.
85
-
for 15 min, 18-20% activity depending on purification procedure Enterobacter sp.
100
-
for 15 min, total loss of activity Enterobacter sp.