Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.20 extracted from

  • Houser, W.M.; Butterer, A.; Addepalli, B.; Limbach, P.A.
    Combining recombinant ribonuclease U2 and protein phosphatase for RNA modification mapping by liquid chromatography-mass spectrometry (2015), Anal. Biochem., 478, 52-58 .
    View publication on PubMed

Application

Application Comment Organism
analysis bacteriophage lambda protein phosphatase and recombinant RNase U2 can be used to generate RNase digestion products amenable to liquid chromatography-tandem mass spectrometry analysis, which can be used to determine the presence and location of modified ribonucleosides in RNA samples synthetic construct

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the synthetic gene for RNase U2 in Escherichia coli synthetic construct

Organism

Organism UniProt Comment Textmining
synthetic construct KP779641 derived from Ustilago sphaerogena
-

Purification (Commentary)

Purification (Comment) Organism
-
synthetic construct

Synonyms

Synonyms Comment Organism
RNase U2
-
synthetic construct